Increased ascorbate biosynthesis does not improve nitrogen fixation nor alleviate the effect of drought stress in nodulated Medicago truncatula plants
Fecha
2021Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Identificador del proyecto
Impacto
|
10.3389/fpls.2021.686075
Resumen
Legume plants are able to establish nitrogen-fixing symbiotic relations with Rhizobium
bacteria. This symbiosis is, however, affected by a number of abiotic constraints,
particularly drought. One of the consequences of drought stress is the overproduction
of reactive oxygen (ROS) and nitrogen species (RNS), leading to cellular damage and,
ultimately, cell death. Ascorbic acid (AsA), also know ...
[++]
Legume plants are able to establish nitrogen-fixing symbiotic relations with Rhizobium
bacteria. This symbiosis is, however, affected by a number of abiotic constraints,
particularly drought. One of the consequences of drought stress is the overproduction
of reactive oxygen (ROS) and nitrogen species (RNS), leading to cellular damage and,
ultimately, cell death. Ascorbic acid (AsA), also known as vitamin C, is one of the
antioxidant compounds that plants synthesize to counteract this oxidative damage. One
promising strategy for the improvement of plant growth and symbiotic performance
under drought stress is the overproduction of AsA via the overexpression of enzymes
in the Smirnoff-Wheeler biosynthesis pathway. In the current work, we generated
Medicago truncatula plants with increased AsA biosynthesis by overexpressing MtVTC2,
a gene coding for GDP-L-galactose phosphorylase. We characterized the growth and
physiological responses of symbiotic plants both under well-watered conditions and
during a progressive water deficit. Results show that increased AsA availability did not
provide an advantage in terms of plant growth or symbiotic performance either under
well-watered conditions or in response to drought. [--]
Materias
Ascorbic acid,
Legume,
Symbiosis,
Water deficit,
Antioxidant
Editor
Frontiers Media
Publicado en
Frontiers in Plant Science June 2021, Volume 12, Article 686075
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. Institute for Multidisciplinary Research in Applied Biology - IMAB
Versión del editor
Entidades Financiadoras
This work had been funded by the Spanish Ministry of Science and Innovation-European Regional Development Fund (grant RTI2018-094623-B-C22) and the Government of Navarra (project PC112-113 LEGUSI). EL was a Ramón y Cajal fellow (RYC2018-023867-I) and LC-P and MR were Formación de Personal Investigador fellows from the Spanish Ministry of Economy and Competitiveness (BES-2015-074411 and BES-2012-059972, respectively).
Aparece en las colecciones
Los documentos de Academica-e están protegidos por derechos de autor con todos los derechos reservados, a no ser que se indique lo contrario.
La licencia del ítem se describe como © 2021 Cobos-Porras, Rubia, Huertas, Kum, Dalton, Udvardi, Arrese-Igor and Larrainzar. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.