Katsukawa, ShihoShishido, Kazuo2018-02-142018-02-14200684-9769-107-5https://academica-e.unavarra.es/handle/2454/27213Comunicación presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.We cloned and sequenced a recQ gene homologue from Lentinula edodes. This gene, named Le.recQ, was found to have a coding capacity of 945 amino acids (aa). The deduced Le.RECQ protein was clearly smaller than other fungal RecQ proteins such as Neurospora crassa QDE3 (1955 aa), Schizosaccharomyces pombe Rqh1 (1328 aa), and Saccharomyces cerevisiae SGS1 (1447 aa). It exhibited the highest homology to the Arabidopsis thaliana RecQl4A protein (1182 aa) in its size and aa sequence. Northern-blot analysis showed that the Le.recQ gene is transcribed at similar levels during mycelial development in L. edodes fruiting-body formation. The L. edodes dikaryotic mycelial cells were found to contain a clearly larger amount of Le.recQ transcript than the L. edodes two compatible monokaryotic mycelial cells. Results in situ RNA-RNA hybridization showed that subhymenium and outer region of trama contain larger amounts of Le.recQ transcript. Expression of Le.recQ cDNA in S. cerevisiae might partially complement defects associated with the loss of its homologue S. cerevisiae SGS1 gene.11 p.application/pdfeng© Autores; Universidad Pública de Navarra. Esta publicación no puede ser reproducida, almacenada o transmitida total o parcialmente, sea cual fuere el medio y el procedimiento, incluidas las fotocopias, sin permiso previo concedido por escrito por los titulares del copyright.Lentinula edodesLe.recQ geneinfo:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/openAccess