Eugui Arrizabalaga, DanielFernández San Millán, AliciaVelasco, PabloVeramendi Charola, JonRodríguez, Víctor ManuelPoveda Arias, Jorge2025-06-192025-06-192025-05-27Eugui, D., Fernández San Millán, A., Velasco, P., Veramendi, J., Rodríguez, V. M., Poveda, J. (2025) Broccoli (Brassica oleracea var. italica) biomass as a resource for obtaining glucosinolate extracts to control postharvest fungal diseases. Journal of Plant Diseases and Protection, 132(3), 1-8. https://doi.org/10.1007/s41348-025-01099-w.1861-382910.1007/s41348-025-01099-whttps://academica-e.unavarra.es/handle/2454/54269Broccoli (Brassica oleracea var. italica) is a crop of great agronomic and economic importance worldwide. Because its edible parts are the inflorescences, large quantities of non-commercial biomass are produced each year in the field and in the food industry. In order to develop a circular economy around the broccoli crop, the present work develops glucosinolates (GSL) extracts with antimicrobial capacity for postharvest use in tomato, apple and table white grape against fungal diseases produced by the pathogens Botrytis cinerea, Alternaria alternata and Penicillium expansum. GSL extracts from organic crop management reported a higher content of GSLs than conventional management. These extracts are not effective in the control of A. alternata and P. expansum, possibly due to the absence of sinigrin. Furthermore, the extracts were ineffective in the control of B. cinerea on table white grapes, possibly due to the non-climacteric fruit condition and an absence in the induction of ethylene-mediated plant defenses. However, intact GSL extracts were effective in controlling B. cinerea on apple, while the addition of myrosinase enzyme caused effectiveness also on tomato and apple. Therefore, obtaining GSL extracts with biopesticidal capacity against B. cinerea in postharvest could be a circular economy strategy for broccoli agriculture and industry.application/pdfeng© The Author(s) 2025. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.Tomato CherryBotrytis cinereaMyrosinase enzymeGlucosinolate hydrolysis productsGlucobrassicininfo:eu-repo/semantics/article2025-06-19info:eu-repo/semantics/openAccess