Aguilera, SeleneTorre Zavala, Susana de laHernández Flores, José LuisMurillo Martínez, JesúsBravo, JaimeÁlvarez Morales, Ariel2014-05-142014-05-1420121932-620351010.1371/journal.pone.0046815https://academica-e.unavarra.es/handle/2454/10523Incluye material complementarioThe bacterium Pseudomonas syringae pv. phaseolicola produces phaseolotoxin in a temperature dependent manner, being optimally produced between 18 degrees C and 20 degrees C, while no detectable amounts are present above 28 degrees C. Phaseolotoxin is an effective inhibitor of ornithine carbamoyltransferase (OCTase) activity from plant, mammalian and bacterial sources and causes a phenotypic requirement for arginine. To protect the cell from its own toxin, P. syringae pv. phaseolicola synthesizes a phaseolotoxin-resistant OCTase (ROCT). The ROCT is the product of the argK gene and is synthesized only under conditions leading to phaseolotoxin synthesis. The argK gene is included in a chromosomal fragment named Pht cluster, which contains genes involved in the synthesis of phaseolotoxin. The aim of the present work was to investigate the possible involvement of other genes included in the Pht cluster in the regulation of gene argK. We conducted transcriptional analyses of argK in several mutants unable to produce phaseolotoxin, transcriptional fusions and electrophoretic mobility shift assays, which allowed us to determine that genes phtABC, located within the Pht cluster, participate in the transcriptional repression of gene argK at temperatures not permissive for phaseolotoxin biosynthesis. This repression is mediated by a protein present in both toxigenic and nontoxigenic strains of P. syringae and in E. coli, and requires the coordinated participation of phtA, phtB and phtC products in order to carry out an efficient argK repression.application/pdfapplication/zipeng© 2012 Aguilera et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Two ornithine carbamoyltransferases [2 ornithine carbamoyltransferases]Halo blightPhytotoxin coronatineBeta glucuronidaseToxinProductionBiosynthesisClusterProteinTemperatureRegulatorArtículo / ArtikuluaAcceso abierto / Sarbide irekiainfo:eu-repo/semantics/openAccess