Pereira, CatiaMontalbán, Itziar A.Goicoa Mangado, TomásUgarte Martínez, María DoloresCorreia, SandraCanhoto, Jorge M.Moncaleán, Paloma2018-09-042018-09-0420172171-9845 (Electronic)10.5424/fs/2017263-11436https://academica-e.unavarra.es/handle/2454/30428Aim of the study: The effect of physical and chemical conditions at proliferation stage was evaluated in order to elucidate if this stage is the determinant phase to induce a marked effect in Pinus halepensis somatic embryogenesis. Area of study: The study was conducted in research laboratories of Neiker (Arkaute, Spain). Material and methods: Pinus halepensis embryonal masses from ten embryogenic cell lines subjected to nine treatments (tissues cultured at three temperatures on media supplemented with three agar concentrations) at proliferation stage. Main results: Significant differences were observed among different proliferation conditions months later at the end of maturation, germination and acclimatization stages. Research highlights: Aleppo pine embryonal masses are cultured under standard conditions on a culture medium supplemented with 4.5 g/L Gelrite® at 23ºC. However, better results in terms of plantlet production can be obtained proliferating the embryonal masses at 18ºC in a culture media with significantly lower water availability.4 p.application/pdfeng© 2017 INIA. This is an open access article distributed under the terms of the Creative Commons Attribution (CC-by) Spain 3.0 License.Embryogenic cell linesPinus halepensisSomatic embryosWater availabilityinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess