Buezo Bravo, JavierEsteban Terradillos, RaquelCornejo Ibergallartu, AlfonsoLópez Gómez, PedroMarino Bilbao, DanielChamizo Ampudia, AlejandroGil Idoate, María JoséMartínez Merino, VíctorMorán Juez, José Fernando2019-08-192021-10-0120190168-9452 (Print)1873-2259 (Electronic)10.1016/j.plantsci.2019.110176https://academica-e.unavarra.es/handle/2454/34425Indole-3-acetaldoxime (IAOx) is a particularly relevant molecule as an intermediate in the pathway for tryptophan-dependent auxin biosynthesis. The role of IAOx in growth-signalling and root phenotype is poorly studied in cruciferous plants and mostly unknown in non-cruciferous plants. We synthesized IAOx and applied it to M. truncatula plants grown axenically with NO3-, NH4+ or urea as the sole nitrogen source. During 14 days of growth, we demonstrated that IAOx induced an increase in the number of lateral roots, especially under NH4+ nutrition, while elongation of the main root was inhibited. This phenotype is similar to the phenotype known as “superroot” previously described in SUR1- and SUR2-defective Arabidopsis mutants. The effect of IAOx, IAA or the combination of both on the root phenotype was different and dependent on the type of N-nutrition. Our results also showed the endogenous importance of IAOx in a legume plant in relation to IAA metabolism, and suggested IAOx long-distance transport depending on the nitrogen source provided. Finally, our results point out to CYP71A as the major responsible enzymes for IAA synthesis from IAOx.31 p.application/pdfapplication/zipeng© 2019 Elsevier B.V. This manuscript version is made available under the CC-BY-NC-ND 4.0.AmmoniumAuxinIndole-3-acetaldoximeNitrateOximesPhenotypeSuperrootRootUreaCYP71AArtículo / ArtikuluaAcceso abierto / Sarbide irekiainfo:eu-repo/semantics/openAccess