Eydallin, GustavoMontero Macarro, ManuelAlmagro Zabalza, GoizederSesma Pascual, María TeresaViale, Alejandro M.Muñoz Pérez, Francisco JoséRahimpour, MehdiBaroja Fernández, EdurnePozueta Romero, Javier2014-06-112014-06-1120101340-283864610.1093/dnares/dsp028https://academica-e.unavarra.es/handle/2454/10807Using a systematic and comprehensive gene expression library (the ASKA library), we have carried out a genome-wide screening of the genes whose increased plasmid-directed expression affected glycogen metabolism in Escherichia coli. Of the 4123 clones of the collection, 28 displayed a glycogen-excess phenotype, whereas 58 displayed a glycogen-deficient phenotype. The genes whose enhanced expression affected glycogen accumulation were classified into various functional categories including carbon sensing, transport and metabolism, general stress and stringent responses, factors determining intercellular communication, aggregative and social behaviour, nitrogen metabolism and energy status. Noteworthy, one-third of them were genes about which little or nothing is known. We propose an integrated metabolic model wherein E. coli glycogen metabolism is highly interconnected with a wide variety of cellular processes and is tightly adjusted to the nutritional and energetic status of the cell. Furthermore, we provide clues about possible biological roles of genes of still unknown functions.application/pdfeng© The Author 2010. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.ASKA libraryCarbohydrate metabolismFunctional genomicsADP glucose pyrophosphorylaseAdenylate energy chargeBacterialGlycogenPhosphotransferase systemTyrosine phosphorylationProtein phosphorylationMulticellular behaviorStringent responseCarbon metabolismBiofilm formationArtículo / ArtikuluaAcceso abierto / Sarbide irekiainfo:eu-repo/semantics/openAccess