Dorado Morales, PedroMartínez, IgorRivero Buceta, VirginiaDíaz, EduardoBähre, HeikeLasa Uzcudun, ÍñigoSolano Goñi, Cristina2021-06-232021-06-2320201751-790710.1111/1751-7915.13689https://academica-e.unavarra.es/handle/2454/40014Incluye material complementarioBacterial biofilms provide high cell density and a superior adaptation and protection from stress conditions compared to planktonic cultures, making them a very promising approach for bioremediation. Several Rhodococcus strains can desulfurize dibenzothiophene (DBT), a major sulphur pollutant in fuels, reducing air pollution from fuel combustion. Despite multiple efforts to increase Rhodococcus biodesulfurization activity, there is still an urgent need to develop better biocatalysts. Here, we implemented a new approach that consisted in promoting Rhodococcus erythropolis biofilm formation through the heterologous expression of a diguanylate cyclase that led to the synthesis of the biofilm trigger molecule cyclic di-GMP (c-di-GMP). R. erythropolis biofilm cells displayed a significantly increased DBT desulfurization activity when compared to their planktonic counterparts. The improved biocatalyst formed a biofilm both under batch and continuous flow conditions which turns it into a promising candidate for the development of an efficient bioreactor for the removal of sulphur heterocycles present in fossil fuels.15 p.application/pdfapplication/zipeng© 2020 The Authors. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use,distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.Rhodococcus erythropolisBiofilm formationDibenzothiophene (DBT)DesulfurizationC-di-GMPinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess