Miranda Jiménez, CarlosIrisarri, P.Arellano Zapatero, JuliaBielsa, F. J.Valencia Leoz, AnaUrrestarazu Vidart, JorgePina, AnaSantesteban García, GonzagaCastel, L.Errea, Pilar2023-09-082024-03-302023Miranda, C., Irisarri, P., Arellano-Zapatero, J., Bielsa, F. J., Valencia-Leoz, A., Urrestarazu-Vidart, J., Pina, A., Santesteban-García, L. G., Castel, L., Errea, P. (2023) Assessment of flesh browning diversity in apple germplasm collections phenotyped by image analysis. En Bus, V., Causse M. (Eds.), XXXI International Horticultural Congress (IHC2022): international symposium on breeding and effective use of biotechnology and molecular tools in horticultural crops (pp. 1-6). ISHS. https://doi.org/10.17660/ActaHortic.2023.1362.64.978-94-62613-61-410.17660/ActaHortic.2023.1362.64https://academica-e.unavarra.es/handle/2454/46252Enzymatic flesh browning (EB) is one of the major problems affecting the quality and limiting the shelf life of minimally processed fruit. Traditionally, EB has been measured objectively using colourimeters. However, colourimeters are not suitable for phenotyping large quantities of fruit samples as they measure just one small area of a sample at a time, which hampers the acquisition of representative measurements and renders them time-consuming and costly. Previous research has shown that image analysis of digital photographs could be a viable alternative to obtain colour information of the entire surface of samples for large scale phenotyping, but to date there are no references for its practical application. The aim of this work was to assess the diversity in EB in a large set of cultivars phenotyped using digital photographs and a high-throughput analytical system based on image analysis developed by our team. A set of 143 cultivars from 104 genotypes, including modern references (16 cultivars) and traditional Spanish cultivars from UPNA (67 cultivars) and CITA (60 cultivars) germplasm collections was analysed in 2020 and 2021. The traditional cultivars were part of the core collection, which optimizes the representativeness of the genetic variation of apples preserved in Spanish collections. EB was evaluated in 10 fruits per cultivar and photographed at regular intervals from just after cutting to one hour later. A wide range of EB intensities was observed, with up to 20-fold differences between cultivars, which could be classified into five levels using two indices. The time at which EB was evaluated (30 or 60 min after slicing) had little influence on the classification. Traditional cultivars with low or very low EB were found to be comparable to those of references with less EB. The results show the potential of traditional germplasm to diversify the varietal offer and introduce new traits in apple breeding.application/pdfengBrowning degreeGenetic resourcesMalus × domestica BorkhPhenotypingContribución a congreso / Biltzarrerako ekarpena2023-09-08Acceso abierto / Sarbide irekiainfo:eu-repo/semantics/openAccess