Cordero, P.Campión, J.Milagro Yoldi, F. I.Marzo Pérez, FlorencioMartínez, J. A.2014-06-102014-06-1020081476-511X71710.1186/1476-511X-7-49https://academica-e.unavarra.es/handle/2454/10790The glyoxylate cycle, which is well characterized in higher plants and some microorganisms but not in vertebrates, is able to bypass the citric acid cycle to achieve fat-to-carbohydrate interconversion. In this context, the hydrodynamic transfer of two glyoxylate cycle enzymes, such as isocytrate lyase (ICL) and malate synthase (MS), could accomplish the shift of using fat for the synthesis of glucose. Therefore, 20 mice weighing 23.37 +/- 0.96 g were hydrodinamically gene transferred by administering into the tail vein a bolus with ICL and MS. After 36 hours, body weight, plasma glucose, respiratory quotient and energy expenditure were measured. The respiratory quotient was increased by gene transfer, which suggests that a higher carbohydrate/lipid ratio is oxidized in such animals. This application could help, if adequate protocols are designed, to induce fat utilization of obesity and diabetes.application/pdfeng© 2008 Cordero et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Tail vein injectionPlasmid deliveryLiverExpressionRatsUCP1ManipulationMechanismObesityMiceinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess