Muñoz, RobertoSantamaría Martínez, EnriqueRubio, IdoyaAusín, KarinaLabarga Gutiérrez, Alberto2020-12-022020-12-0220181422-006710.3390/ijms19020498https://academica-e.unavarra.es/handle/2454/38817Thrombotic material retrieved from acute ischemic stroke (AIS) patients represents a valuable source of biological information. In this study, we have developed a clinical proteomics workflow to characterize the protein cargo of thrombi derived from AIS patients. To analyze the thrombus proteome in a large-scale format, we developed a workflow that combines the isolation of thrombus by endovascular thrombectomy and peptide chromatographic fractionation coupled to mass-spectrometry. Using this workflow, we have characterized a specific proteomic expression profile derived from four AIS patients included in this study. Around 1600 protein species were unambiguously identified in the analyzed material. Functional bioinformatics analyses were performed, emphasizing a clustering of proteins with immunological functions as well as cardiopathy-related proteins with blood-cell dependent functions and peripheral vascular processes. In addition, we established a reference proteomic fingerprint of 341 proteins commonly detected in all patients. Protein interactome network of this subproteome revealed protein clusters involved in the interaction of fibronectin with 14-3-3 proteins, TGFβ signaling, and TCP complex network. Taken together, our data contributes to the repertoire of the human thrombus proteome, serving as a reference library to increase our knowledge about the molecular basis of thrombus derived from AIS patients, paving the way toward the establishment of a quantitative approach necessary to detect and characterize potential novel biomarkers in the stroke field.17 p.application/pdfapplication/zipeng© 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access_x000D_ article distributed under the terms and conditions of the Creative Commons Attribution_x000D_ (CC BY) license.Ischemic strokeProteomicsBiomarkersEndovascular therapyThrombusinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess