Otero Tuárez, Víctor OswaldoCarrión Granda, XimenaFernández Pan, IdoyaFernández García, TeresaMaté Caballero, Juan Ignacio2018-11-082019-10-1020190950-5423 (Print)1365-2621 (Electronic)10.1111/ijfs.14003https://academica-e.unavarra.es/handle/2454/31317Two distinct strategies were combined to preserve fresh fish (Merluccius merluccius) under refrigeration at 4 °C for 12 days: (i) the application of an antimicrobial edible coating enriched with oregano essential oil (OEO) or carvacrol (CV) and (ii) the reduction of initial microbial load by good handling practise and the use of sodium hypochlorite (NaOCl). The action of antimicrobial coatings alone retarded the growth of Enterobacteriaceae, lactic acid bacteria (LAB) and H2S producing bacteria on fish samples. The reduction of initial microbial load by itself only affected the evolution of LAB, but not the rest of the bacterial groups. When using both techniques combined, edible antimicrobial coatings were significantly more effective with additional and significant delays in the growth of mesophilic, psychrotrophic and Pseudomonas bacteria. Thus, the use of both strategies combined resulted in a reduction of the counts of all bacterial groups after 12 days of storage which ranged from 1.5 log and 8 log, in Pseudomonas and H2S producing bacteria, respectively. Moreover, no significant differences were observed when comparing the microbiological evolution of samples treated with OEO compared to those only treated with CV.application/pdfeng© 2018 Institute of Food Science and TechnologyAntimicrobial edible coatingOregano essential oilInitial microbial loadNaOClRefrigerated hakeWhey protein isolateinfo:eu-repo/semantics/articleAcceso abierto / Sarbide irekiainfo:eu-repo/semantics/openAccess