Valle Turrillas, JaioneBurgui Erice, SaioaLangheinrich, DeniseGil Puig, CarmenSolano Goñi, CristinaToledo Arana, AlejandroHelbig, RalfLasagni, AndrésLasa Uzcudun, Íñigo2016-11-042016-11-042015https://academica-e.unavarra.es/handle/2454/22611Esta es la versión no revisada del artículo: Valle, J., Burgui, S., Langheinrich, D., Gil, C., Solano, C., Toledo-Arana, A., et al. (2015). Evaluation of Surface Microtopography Engineered by Direct Laser Interference for Bacterial Anti-Biofouling. Macromolecular Bioscience, 15(8), 1060–1069. Se puede consultar la versión publicada en http://doi.org/10.1002/mabi.201500107Biofilm formation by bacterial pathogens on the surface of medical and industrial settings is a 25 serious health problem. Modification of the biomaterial surface topography is a promising 26 strategy to prevent bacterial attachment and biofilm development. However, fabrication of 27 functional biomaterials at large scale with periodic network-topology is still problematic. In this 28 study, we use direct laser interference (DLIP), an easily scalable process, to modify polystyrene 29 surface (PS) topography at sub-micrometer scale. The resulting structure surfaces were 30 interrogated for their capacity to prevent adhesion and biofilm formation of the major human 31 pathogen Staphylococcus aureus. The results revealed that three-dimensional micrometer 32 periodic structures on PS have a profound impact on bacterial adhesion capacity. Thus, line- 33 and pillar-like topographical patterns enhanced S. aureus adhesion, whereas complex lamella 34 microtopography reduced S. aureus adhesion both in static and continuous flow culture 35 conditions. Interestingly, lamella-like textured surfaces retained the capacity to inhibit S. aureus 36 adhesion both when the surface is coated with human serum proteins in vitro and when the 37 material is implanted subcutaneously in a foreign-body associated infection model. Our results 38 establish that the DLIP technology can be used to functionalize polymeric surfaces for the 39 inhibition of bacterial adhesion to surfaces.application/pdfengBacterial adhesionBiofilmsStaphylococcus aureusDirect laser interferenceinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess