Razquin, CristinaFernández Irigoyen, JoaquínBarrio-López, María TeresaLópez, BegoñaRavassa, SusanaRamos, PabloMacías-Ruiz, RosaIbáñez Criado, AliciaSantamaría Martínez, EnriqueGoñi, LeticiaCastellanos, EduardoIbáñez Criado, José LuisTercedor, LuisGarcía-Bolao, IgnacioMartínez González, Miguel ÁngelAlmendral, JesúsRuiz Canela, Miguel2025-06-302025-06-302025-01-24Razquin, C., Fernández-Irigoyen, J., Barrio-López, M. T., López, B., Ravassa, S., Ramos, P., Macías-Ruiz, R., Ibañez Criado, A., Santamaría, E., Goni, L., Castellanos, E., Ibáñez Criado, J. L., Tercedor, L., García-Bolao, I., Martínez-González, M. A., Almendral, J., Ruiz-Canela, M. (2025) Proteomics and recurrence of atrial fibrillation: a pilot study nested in the PREDIMAR trial. Lifestyle Genomics, 18(1), 52-58. https://doi.org/10.1159/000543639.2504-316110.1159/000543639https://academica-e.unavarra.es/handle/2454/54349Introduction: Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia worldwide. Although catheter ablation is the most efficacious therapy, relapses occur frequently (30%) in the first year after ablation. Novel biomarkers of recurrence are needed for a better prediction of recurrence and management of AF. In this pilot study, we aimed to analyze the baseline proteome of subjects included in a case-control study to find differential proteins associated with AF recurrence. Methods: Baseline serum proteomics (354 proteins) data from 16 cases (recurrent AF) and 17 controls (non-recurrent) were obtained using MS/MS analysis. A false discovery rate was performed using a nonlinear fitting method for the selection of proteins. Logistic regression models were used to further analyze the association between differentially expressed proteins and AF recurrence. Results: Ten proteins were differentially represented in cases vs. controls. Two were upregulated in the cases compared to the controls: keratin type I cytoskeletal 17 (Fold-change [FC] = 2.14; p = 0.017) and endoplasmic bifunctional protein (FC = 1.65; p = 0.032). Eight were downregulated in the cases compared to the controls: C4bpA (FC = 0.64; p = 0.006), coagulation factor XI (FC = 0.83; p = 0.011), CLIC1 (FC = 0.62; p = 0.017), haptoglobin (FC = 0.37; p = 0.021), Ig alpha-2 chain C region (FC = 0.49; p = 0.022), C4bpB (FC = 0.73; p = 0.028), N-acetylglucosamine-1- phosphotransferase subunit gamma (FC = 0.61; p = 0.033), and platelet glycoprotein Ib alpha chain (FC = 0.84; p = 0.038). Conclusion: This pilot study identifies ten differentially expressed serum proteins associated with AF recurrence, offering potential biomarkers for improved prediction and management.application/pdfapplication/zipeng© 2025 The Author(s). Published by S. Karger AG, Basel. This article is licensed under the Creative Commons AttributionNonCommercial 4.0 International License (CC BY-NC), usage and distributionfor commercial purposes requires written permission.ProteomicsAtrial fibrillationRecurrencePREDIMARinfo:eu-repo/semantics/article2025-06-30info:eu-repo/semantics/openAccess