Osés Recalde, MaddiMedrano Echeverría, MaríaMargareto, JavierPortillo, María P.Aguilera, Concepción MaríaAltmäe, SigneLabayen Goñi, Idoia2023-01-192023-01-192022Oses, M., Medrano, M., Margareto Sanchez, J., Portillo, M. P., Aguilera, C. M., Altmäe, S., & Labayen, I. (2022). Peripheral blood mononuclear cells‐expressed miRNA profiles derived from children with metabolic‐associated fatty liver disease and insulin resistance. Pediatric Obesity, 17(12). https://doi.org/10.1111/ijpo.129662047-630210.1111/ijpo.12966https://academica-e.unavarra.es/handle/2454/44591Background: miRNA have been proposed as potential biomarkers of metabolic diseases. Objectives: To identify potential miRNA biomarkers of early metabolic-associated fatty liver disease (MAFLD) and/or insulin resistance (IR) in preadolescent children. Methods: A total of 70 preadolescents, aged 8.5–12 years old participated in the study. Hepatic fat was assessed by magnetic resonance imaging. Fasting blood biochemical parameters were measured and HOMA-IR calculated. Peripheral blood mononuclear cells (PBMC)-derived miRNA profiles associated with MAFLD (≥5.5% hepatic fat) and IR (HOMA-IR ≥2.5) were identified using untargeted high-throughput miRNAs sequencing (RNA-seq). Results: A total of 2123 PBMC-derived miRNAs were identified in children with (21.4%) or without MAFLD. Among them, hsa-miR-143-3p, hsa-miR-142-5p and hsamiR-660-5p were up-regulated, and p-hsa-miR-247, hsa-let-7a-5p and hsa-miR6823-3p down-regulated. Importantly, children with MAFLD had consistently higher miR-660-5p expression levels than their peers without it (p < 0.01), regardless of weight status. A total of 2124 PBMC-derived miRNA were identified in children with IR (28.6%) versus children without IR, where thirteen of them were dysregulated (p < 0.05) in children with IR. In addition, children with IR showed higher levels of miR-374a-5p and miR-190a-5p (p < 0.01) and lower levels of miR-4284 and miR4791 (p < 005), than their peers without IR in both the whole sample and in those with overweight or obesity. Conclusions: Our study results suggest circulating miR-660-5p as a potential biomarker of the presence of MAFLD in preadolescent children while circulating miR320a, miR-142-3p, miR-190a-5p, miR-374a-5p and let-7 family miRNAs could serve as potential biomarkers of IR in children.application/pdfapplication/zipeng© 2022 The Authors. This is an open access article under the terms of theCreative Commons Attribution-NonCommercialLicense, which permits use, distribution and reproduction in anymedium, provided the original work is properly cited and is not used for commercial purposes.ChildrenFatty liverInsulin resistanceMetabolic diseasesMiRNAObesityArtículo / Artikulua2023-01-19Acceso abierto / Sarbide irekiainfo:eu-repo/semantics/openAccess