Zubiate Orzanco, PabloUrrutia Azcona, AitorRuiz Zamarreño, CarlosEgea Urra, JosuneFernández Irigoyen, JoaquínGiannetti, AmbraBaldini, FrancescoDíaz Lucas, SilviaMatías Maestro, IgnacioArregui San Martín, Francisco JavierSantamaría Martínez, EnriqueChiavaioli, FrancescoDel Villar, Ignacio2020-03-062020-03-0620192590-137010.1016/j.biosx.2019.100026https://academica-e.unavarra.es/handle/2454/36429D-dimer is a useful diagnostic biomarker for deep vein thrombosis or pulmonary embolism, collectively referred to as venous thromboembolism (VTE). The ability to detect in real-time the amount of D-dimer with a fast and reliable method is a key step to anticipate the appearance of these diseases. Here, the results of a highly specific and sensitive biosensor for the detection of D-dimer based on lossy mode resonance in fiber optics are presented. The unique features of specialty fibers in light management integrated with microfluidics allow detecting D-dimer in human serum with a detection limit of 100 ng/mL, a value 5-fold below the clinical cutoff value. Comparison of the results achieved with mass-spectrometry-based proteomics, which allows for the identification of beta- and gamma-chains of fibrinogen, demonstrates the ability of our platform to specifically (>90%) recognize D-dimer. Therefore, this technology potentially represents a paradigm shift in the development of a simple, high-specificity and label-free biosensing platform, which can be applied to speed up diagnostic healthcare processes of venous thromboembolism toward an early diagnostic and personalized treatment system.10 p.application/pdfeng© 2019 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license.Optical biosensorLossy mode resonanceD-dimerVenous thromboembolismNanocoated fiberinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessAcceso abierto / Sarbide irekia