Simoes da Mota, Ana ClaudiaBarré, PierreBaudin, FrançoisPoch, Rosa MaríaBruni, ElisaAntón Sobejano, RodrigoEnrique Martín, AlbertoVirto Quecedo, Íñigo2024-09-192024-09-192024Simões-Mota, A., Barré, P., Baudin, F., Poch, R. M., Bruni, E., Anton, R., Enrique, A., Virto, I. (2024) Effects of long-term sewage sludge addition to a calcareous soil on soil organic C fractions and soil functions. Geoderma, 445, 1-11. https://doi.org/10.1016/j.geoderma.2024.116868.0016-706110.1016/j.geoderma.2024.116868https://academica-e.unavarra.es/handle/2454/51663Soil organic matter (SOM) is a recognized carbon reservoir and paramount in soil functioning and agrosystems productivity. Different management strategies have been established to enhance SOM in arable soils, and one is the application of exogenous organic matter (EOM). Despite significant efforts in recent years, the consequences of EOM addition on increasing soil organic C (SOC) storage and stability in different pedoclimatic contexts remain incompletely understood. In this study, we evaluated the effect of long-term (28 y) sewage sludge (SS) addition to a calcareous soil supporting rainfed extensive crops in a Mediterranean sub-humid area in terms of SOC stabilization and distribution among functional fractions. To that end, we studied total SOM storage and soil fractions in a long-term experiment comparing 4 different doses of SS with mineral fertilization and no-fertilization controls. We compared the concentration, storage and distribution of C using two different fractionation methods (particulate vs. mineral-associated, defined by granulodensimetric fractionation, and active C vs. stable C defined by Rock-Eval® thermal analysis coupled to the machine learning PARTYsoc v2.0 model) on the tilled layer (0–30 cm). Three soil functioning indicators (crops yield, soil microbial biomass C and aggregate stability) were also quantified. We found that SS application, which slightly increased SOC concentration when SS was added, resulted in a net SOC stock gain only with the highest dose used (80 tons/ha), compared to mineral fertilization, suggesting that most of the C added was mineralized. An uneven response of soil fractions was however detected. The coarsest heavy physical fractions > 250 μm and 50–250 μm in size were the most enriched in their C concentration with SS addition, whereas SOC was mostly accumulated as mineral-associated C in the silt-size (2–50 μm) and the 50–250 μm fractions. Regarding thermal fractionation, SS treatments showed C gains between 57 % and 35 % in the active C pool, compared to mineral fertilization. This can be explained considering that the accumulated SOM in mineral-associated fractions corresponded to mean-residence times in the order of 20–40 years. The consequences on soil functioning indicators were not directly related to the amount of SOC stored in the soil, as the highest SOC gains corresponded to the highest dose, but not the highest yields, and some negative correlations were observed between SOC fractions and soil structural stability and microbial biomass. This can be related to some deleterious effects of excessive SS application reducing yields, the soil biological activity and soil structure, and represents an example of the need to decouple the assessment of soil health from that of SOC storage for certain soil management practices. The overall evaluation of the net consequences of long-term SS application indicated that low doses (10 tons/ha) seem a better choice, as they resulted in the highest efficiency in C incorporation, and in a slightly greater increase in SOC concentration than intermediate doses, and equal yields than mineral fertilization, but did not have the negative effects observed in soil functioning with higher doses.application/pdfapplication/mswordeng© 2024 The Author(s). This is an open access article under the CC BY-NC-ND licenseCalcareous soilsOrganic matter fractionationRock-Eval®Sewage sludgeSoil organic Cinfo:eu-repo/semantics/article2024-09-19info:eu-repo/semantics/openAccess