Listar por autor UPNA "Lasa Uzcudun, Íñigo"
Mostrando ítems 1-20 de 73
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Adenosine diphosphate sugar pyrophosphatase prevents glycogen biosynthesis in Escherichia coli
An adenosine diphosphate sugar pyrophosphatase (ASPPase, EC 3.6.1.21) has been characterized by using Escherichia coli. This enzyme, whose activities in the cell are inversely correlated with the intracellular glycogen ... -
Amyloid structures as biofilm matrix scaffolds
Recent insights into bacterial biofilm matrix structures have induced a paradigm shift toward the recognition of amyloid fibers as common building block structures that confer stability to the exopolysaccharide matrix. ... -
Analysis of the association between polymorphisms in intergenic regions of Staphylococcus aureus genes involved in biofilm formation and periprosthetic joint infections
In this thesis, we have focused on studying variants found in IGRs adjacent to the most important genes involved in S. aureus biofilm formation; the icaADBCR locus, and the genes encoding the family of surface adhesins. ... -
Antibiofilm activity of flavonoids on staphylococcal biofilms through targeting BAP amyloids
The opportunistic pathogen Staphylococcus aureus is responsible for causing infections related to indwelling medical devices, where this pathogen is able to attach and form biofilms. The intrinsic properties given by the ... -
B regulates IS256-mediated Staphylococcus aureus biofilm phenotypic variation
Biofilm formation in Staphylococcus aureus is subject to phase variation, and biofilm-negative derivatives emerge sporadically from a biofilm-positive bacterial population. To date, the only known mechanism for generating ... -
Bap, a biofilm matrix protein of Staphylococcus aureus prevents cellular internalization through binding to GP96 host receptor
The biofilm matrix, composed of exopolysaccharides, proteins, nucleic acids and lipids, plays a well-known role as a defence structure, protecting bacteria from the host immune system and antimicrobial therapy. However, ... -
Bap, a Staphylococcus aureus surface protein involved in biofilm formation
Identification of new genes involved in biofilm formation is needed to understand the molecular basis of strain variation and the pathogenic mechanisms implicated in chronic staphylococcal infections. A biofilm-producing ... -
Base pairing interaction between 5′- and 3′-UTRs controls icaR mRNA translation in Staphylococcus aureus
The presence of regulatory sequences in the 39 untranslated region (39-UTR) of eukaryotic mRNAs controlling RNA stability and translation efficiency is widely recognized. In contrast, the relevance of 39-UTRs in bacterial ... -
Beta-lactam antibiotics induce the SOS response and horizontal transfer of virulence factors in Staphylococcus aureus
Antibiotics that interfere with DNA replication and cell viability activate the SOS response. In Staphylococcus aureus, the antibiotic-induced SOS response promotes replication and high-frequency horizontal transfer ... -
Biofilm dispersion and quorum sensing
Biofilm development and quorum sensing are closely interconnected processes. Biofilm formation is a cooperative group behaviour that involves bacterial populations living embedded in a self produced extracellular matrix. ... -
Biofilm matrix exoproteins induce a protective immune response against Staphylococcus aureus biofilm infection
The Staphylococcus aureus biofilm mode of growth is associated with several chronic infections that are very difficult to treat due to the recalcitrant nature of biofilms to clearance by antimicrobials. Accordingly, there ... -
Biofilm properties in relation to treatment outcome in patients with first-time periprosthetic hip or knee joint infection
Background: periprosthetic joint infections (PJI) are challenging complications following arthroplasty. Staphylococci are a frequent cause of PJI and known biofilm producers. Biofilm formation decreases antimicrobial ... -
Biofilm switch and immune response determinants at early stages of infection
Biofilm development is recognized as a major virulence factor underlying most chronic bacterial infections. When a biofilm community is established, planktonic cells growing in the surroundings of a tissue switch to a ... -
The biofilm-associated surface protein Esp of Enterococcus faecalis forms amyloid-like fibers
Functional amyloids are considered as common building block structures of the biofilm matrix in different bacteria. In previous work, we have shown that the staphylococcal surface protein Bap, a member of the Biofilm-Associated ... -
Calcium inhibits bap-dependent multicellular behavior in Staphylococcus aureus
Bap (biofilm-associated protein) is a 254-kDa staphylococcal surface protein implicated in formation of biofilms by staphylococci isolated from chronic mastitis infections. The presence of potential EF-hand motifs in the ... -
Caracterización del sistema sensorial de dos componentes de Staphylococcus aureus
En este trabajo de tesis hemos evaluado dos aproximaciones diferentes dedicadas a la búsqueda de fármacos que tengan como diana de acción los TCSs. Por un lado, dentro del proyecto 'Nuevas estrategias para el control de ... -
Cloning, nucleotide sequencing, and analysis of the AcrAB-TolC efflux pump of enterobacter cloacae and determination of its involvement in antibiotic resistance in a clinical isolate
Enterobacter cloacae is an emerging clinical pathogen that may be responsible for nosocomial infections. Management of these infections is often difficult, owing to the high frequency of strains that are resistant to ... -
Comparative and functional analysis of 3’ untranslated regions in Staphylococcus species
Una molécula de RNA mensajero (mRNA) está compuesta por una región codificante (CDS) flanqueada por dos regiones no traducidas (UTRs), la 5’UTR y la 3’UTR, respectivamente. En eucariotas, las 3’UTRs son elementos claves ... -
Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus
Our previous studies demonstrated that a putative Staphylococcus aureus glycoprotease (Gcp) is essential for bacterial survival, indicating that Gcp may be a novel target for developing antibacterial agents. However, the ... -
Coordinated cyclic-di-GMP repression of salmonella motility through YcgR and cellulose
Cyclic di-GMP (c-di-GMP) is a secondary messenger that controls a variety of cellular processes, including the switch between a biofilm and a planktonic bacterial lifestyle. This nucleotide binds to cellular effectors in ...