Browsing by UPNA Author "Simón De Goñi, Oihane"
Now showing items 1-20 of 26
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Analagous population structures for two alphabaculoviruses highlight a functional role for deletion mutants
A natural Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) isolate from Florida shares a strikingly similar genotypic composition to that of a natural Spodoptera frugiperda MNPV (SfMNPV) isolate from Nicaragua. ... -
Bacmid expression of granulovirus enhancin En3 accumulates in cell soluble fraction to potentiate nucleopolyhedrovirus infection
Enhancins are metalloproteinases that facilitate baculovirus infection in the insect midgut. They are more prevalent in granuloviruses (GVs), constituting up to 5% of the proteins of viral occlusion bodies (OBs). In ... -
Baculovirus expression and functional analysis of Vpa2 proteins from Bacillus thuringiensis
The mode of action underlying the insecticidal activity of the Bacillus thuringiensis (Bt) binary pesticidal protein Vpa1/Vpa2 is uncertain. In this study, three recombinant baculoviruses were constructed using Bac-to-Bac ... -
Biotechnological development of a new bioinsecticide based on a Helicoverpa armigera nucleopolyhedrovirus from Spain
El taladro del tomate, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae), es una de las principales plagas polífagas de la Península Ibérica. El nucleopoliedrovirus simple de H. armigera (HearSNPV) es un método eficaz ... -
Chrysodeixis chalcites nucleopolyhedrovirus (ChchNPV): natural occurrence and efficacy as a biological insecticide on young banana plants in greenhouse and open-field conditions on the Canary Islands
Chrysodeixis chalcites, an important pest of banana crops on the Canary Islands, is usually controlled by chemical insecticides. The present study aimed to evaluate the efficacy of the most prevalent isolate of the ... -
Chrysodeixis chalcites nucleopolyhedrovirus: a useful component for IPM programs on the Canary Islands banana crops
La platanera (Musa acuminata Colla) es uno de los principales cultivos de las Islas Canarias, representando el 30% de la producción agrícola total del archipiealago. Dicho cultivo se realiza bajo invernaderos de malla ... -
A Chrysodeixis chalcites single-nucleocapsid nucleopolyhedrovirus population from the Canary Islands is genotypically structured to maximize survival
A Chrysodeixis chalcites single-nucleocapsid nucleopolyhedrovirus wild-type isolate from the Canary Islands, Spain, named ChchSNPV-TF1 (ChchTF1-wt), appears to have great potential as the basis for a biological insecticide ... -
Coocclusion of Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) and Helicoverpa armigera multiple nucleopolyhedrovirus (HearMNPV): pathogenicity and stability in homologous and heterologous hosts
Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) is a virulent pathogen of lepidopterans in the genera Heliothis and Helicoverpa, whereas Helicoverpa armigera multiple nu-cleopolyhedrovirus (HearSNPV) is a ... -
Deletion genotypes reduce occlusion body potency but increase occlusion body production in a Colombian Spodoptera frugiperda nucleopolyhedrovirus population
A Colombian field isolate (SfCOL-wt) of Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV) is a mixture of different genotypes. To evaluate the insecticidal properties of the different genotypic variants, 83 ... -
Determinant factors in the production of a co-occluded binary mixture of Helicoverpa armigera alphabaculovirus (HearNPV) genotypes with desirable insecticidal characteristics
A co-occluded binary mixture of Helicoverpa armigera nucleopolyhedrovirus genotypes HearSP1B and HearLB6 at a 1:1 ratio (HearSP1B+HearLB6) was selected for the development of a virus-based biological insecticide, which ... -
Development of a new bioinsecticide based on a Chrysodeixis chalcites nucleopolyhedrovirus from the Canary Islands
Chrysodeixis chalcites (Lepidoptera: Noctuidae) es una plaga importante que causa valiosos daños económicos en los cultivos de platanera de las Islas Canarias. El control efectivo de esta plaga con insecticidas químicos ... -
Effects of several UV-protective substances on the persistence of the insecticidal activity of the Alphabaculovirus of Chrysodeixis chalcites (ChchNPV-TF1) on banana (Musa acuminata, Musaceae, Colla) under laboratory and open-field conditions
Alphabaculovirus of Chrysodeixis chalcites (ChchNPV-TF1) has been investigated as a useful bioinsecticide against C. chalcites (Esper) (Lepidoptera: Noctuidae) in banana crops. This study investigated the effects of several ... -
Expression of a peroral infection factor determines pathogenicity and population structure in an insect virus
A Nicaraguan isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus is being studied as a possible biological insecticide. This virus exists as a mixture of complete and deletion genotypes; the latter depend on ... -
First study on the root endophytic fungus Trichoderma hamatum as an entomopathogen: development of a fungal bioinsecticide against cotton leafworm (Spodoptera littoralis)
Cotton leaf worm (Spodoptera littoralis) is a pest that produces important losses in horticultural and ornamental crops in greenhouse, being classified as quarantine pest A2 by EPPO. One of the strategies proposed to control ... -
Functional importance of deletion mutant genotypes in an insect nucleopolyhedrovirus population
A Nicaraguan isolate of a nucleopolyhedrovirus (SfNIC) that attacks the fall armyworm, Spodoptera frugiperda, survives as a mixture of nine genotypes (SfNIC A to I) that all present genomic deletions, except variant B ... -
Genetic structure of a Spodoptera frugiperda nucleopolyhedrovirus population: high prevalence of deletion genotypes
A Nicaraguan field isolate (SfNIC) of Spodoptera frugiperda nucleopolyhedrovirus was purified by plaque assay on Sf9 cells. Nine distinct genotypes, A to I, were identified by their restriction endonuclease profiles. Variant ... -
Genomic sequences of five Helicoverpa armigera nucleopolyhedrovirus genotypes from Spain that differ in their insecticidal properties
Helicoverpa armigera nucleopolyhedrovirus (HearNPV) has proved effective as the basis for various biological insecticides. Complete genome sequences of five Spanish HearNPV genotypes differed principally in the homologous ... -
Lacanobia oleracea nucleopolyhedrovirus (LaolNPV): a new European species of alphabaculovirus with a narrow host range
During an insect sampling program in alfalfa crops near Montpellier, France in 2011, Lacanobia oleracea larvae were collected that died due to nucleopolyhedrovirus infection (LaolNPV). This virus was subjected to molecular ... -
Mixtures of complete and pif1- and pif2-deficient genotypes are required for increased potency of an insect nucleopolyhedrovirus
The insecticidal potency of a nucleopolyhedrovirus population (SfNIC) that infects Spodoptera frugiperda (Lepidoptera) is greater than the potency of any of the component genotypes alone. Occlusion bodies (OBs) produced ... -
A novel binary mixture of Helicoverpa armigera single nucleopolyhedrovirus genotypic variants has improved insecticidal characteristics for control of cotton bollworms
The genotypic diversity of two Spanish isolates of Helicoverpa armigera single nucleopolyhedrovirus (HearSNPV) was evaluated with the aim of identifying mixtures of genotypes with improved insecticidal characteristics for ...