Browsing by UPNA Author "Lázcoz, Paula"
Now showing items 1-6 of 6
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Analysis of stemness gene expression and CD133 abnormal methylation in neuroblastoma cell lines
Neuroblastoma is the most common extracranial solid tumor in children, accounting for up to 10% of all childhood malignancies. Cellular heterogeneity is a hallmark of this embryonal cancer, as distinct neural crest lineages ... -
Changes in gene expression profiling of apoptotic genes in neuroblastoma cell lines upon retinoic acid treatment
To determine the effect of retinoic acid (RA) in neuroblastoma we treated RA sensitive neuroblastoma cell lines with 9-cis RA or ATRA for 9 days, or for 5 days followed by absence of RA for another 4 days. Both isomers ... -
Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR
Background: We present two melting curve analysis (MCA)-based semiquantitative real time PCR techniques to detect the promoter methylation status of genes. The first, MCA-MSP, follows the same principle as standard MSP ... -
Frequent promoter hypermethylation of RASSF1A and CASP8 in neuroblastoma
Background: Epigenetic alterations and loss of heterozygosity are mechanisms of tumor suppressor gene inactivation. A new carcinogenic pathway, targeting the RAS effectors has recently been documented. RASSF1A, on 3p21.3, ... -
In vitro assessment of the role of p53 on chemotherapy treatments in neuroblastoma cell lines
Neuroblastoma is the most frequent malignant extracranial solid tumor of infancy. The overall objective of this work consists of determining the presence of alterations in the p53/MDM2 /p14ARF signaling pathway in neuroblastoma ... -
Promoter methylation of RASSF1A associates to adult secondary glioblastomas and pediatric glioblastomas
While allelic losses and mutations of tumor suppressor genes implicated in the etiology of astrocytoma have been widely assessed, the role of epigenetics is still a matter of study. We analyzed the frequency of promoter ...