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Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR
(BioMed Central, 2008) Artículo / Artikulua,
Background: We present two melting curve analysis (MCA)-based semiquantitative real time PCR techniques to detect the promoter methylation status of genes. The first, MCA-MSP, follows the same principle as standard MSP ...