Now showing items 1-2 of 2
Detection of methylation in promoter sequences by melting curve analysis-based semiquantitative real time PCR
(BioMed Central, 2008) Artículo / Artikulua,
Background: We present two melting curve analysis (MCA)-based semiquantitative real time PCR techniques to detect the promoter methylation status of genes. The first, MCA-MSP, follows the same principle as standard MSP ...
Frequent promoter hypermethylation of RASSF1A and CASP8 in neuroblastoma
(BioMed Central, 2006) Artículo / Artikulua,
Background: Epigenetic alterations and loss of heterozygosity are mechanisms of tumor suppressor gene inactivation. A new carcinogenic pathway, targeting the RAS effectors has recently been documented. RASSF1A, on 3p21.3, ...