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dc.creatorAguilera, Selenees_ES
dc.creatorTorre Zavala, Susana de laes_ES
dc.creatorHernández Flores, José Luises_ES
dc.creatorMurillo Martínez, Jesúses_ES
dc.creatorBravo, Jaimees_ES
dc.creatorÁlvarez Morales, Arieles_ES
dc.date.accessioned2014-05-14T07:34:50Z
dc.date.available2014-05-14T07:34:50Z
dc.date.issued2012
dc.identifier.issn1932-6203
dc.identifier.other510
dc.identifier.urihttps://hdl.handle.net/2454/10523
dc.descriptionIncluye material complementarioes_ES
dc.description.abstractThe bacterium Pseudomonas syringae pv. phaseolicola produces phaseolotoxin in a temperature dependent manner, being optimally produced between 18 degrees C and 20 degrees C, while no detectable amounts are present above 28 degrees C. Phaseolotoxin is an effective inhibitor of ornithine carbamoyltransferase (OCTase) activity from plant, mammalian and bacterial sources and causes a phenotypic requirement for arginine. To protect the cell from its own toxin, P. syringae pv. phaseolicola synthesizes a phaseolotoxin-resistant OCTase (ROCT). The ROCT is the product of the argK gene and is synthesized only under conditions leading to phaseolotoxin synthesis. The argK gene is included in a chromosomal fragment named Pht cluster, which contains genes involved in the synthesis of phaseolotoxin. The aim of the present work was to investigate the possible involvement of other genes included in the Pht cluster in the regulation of gene argK. We conducted transcriptional analyses of argK in several mutants unable to produce phaseolotoxin, transcriptional fusions and electrophoretic mobility shift assays, which allowed us to determine that genes phtABC, located within the Pht cluster, participate in the transcriptional repression of gene argK at temperatures not permissive for phaseolotoxin biosynthesis. This repression is mediated by a protein present in both toxigenic and nontoxigenic strains of P. syringae and in E. coli, and requires the coordinated participation of phtA, phtB and phtC products in order to carry out an efficient argK repression.en
dc.description.sponsorshipThis work was funded by grants from CONACYT (Consejo Nacional de Ciencia y Tecnología; http://www.conacyt.mx), research grant SEP-2006-C01-49958/24089 to AAM and SA (Postdoctoral scholarship), and from the Spanish Plan Nacional I+D+I grant AGL2008-55311-CO2-01 (Ministerio de Ciencia e Innovación; http://micinn.es/), co financed by FEDER, to JM.en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPlos One, 2012, 7 (10): e46815en
dc.rights© 2012 Aguilera et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.en
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/
dc.subjectTwo ornithine carbamoyltransferases [2 ornithine carbamoyltransferases]en
dc.subjectHalo blighten
dc.subjectPhytotoxin coronatineen
dc.subjectBeta glucuronidaseen
dc.subjectToxinen
dc.subjectProductionen
dc.subjectBiosynthesisen
dc.subjectClusteren
dc.subjectProteinen
dc.subjectTemperatureen
dc.subjectRegulatoren
dc.titleExpression of the gene for resistance to phaseolotoxin (argK) depends on the activity of genes phtABC in Pseudomonas syringae pv. Phaseolicolaen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.affiliationNafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Sailaeu
dc.contributor.affiliationUniversidad Pública de Navarra. Departamento de Producción Agrariaes_ES
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1371/journal.pone.0046815
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/AGL2008-55311en
dc.relation.publisherversionhttps://dx.doi.org/10.1371/journal.pone.0046815
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen


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© 2012 Aguilera et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as © 2012 Aguilera et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.