Fat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genes

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Date
2008Version
Acceso abierto / Sarbide irekia
Type
Artículo / Artikulua
Version
Versión publicada / Argitaratu den bertsioa
Impact
|
10.1186/1476-511X-7-49
Abstract
The glyoxylate cycle, which is well characterized in higher plants and some microorganisms but not in vertebrates, is able to bypass the citric acid cycle to achieve fat-to-carbohydrate interconversion. In this context, the hydrodynamic transfer of two glyoxylate cycle enzymes, such as isocytrate lyase (ICL) and malate synthase (MS), could accomplish the shift of using fat for the synthesis ...
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The glyoxylate cycle, which is well characterized in higher plants and some microorganisms but not in vertebrates, is able to bypass the citric acid cycle to achieve fat-to-carbohydrate interconversion. In this context, the hydrodynamic transfer of two glyoxylate cycle enzymes, such as isocytrate lyase (ICL) and malate synthase (MS), could accomplish the shift of using fat for the synthesis of glucose. Therefore, 20 mice weighing 23.37 +/- 0.96 g were hydrodinamically gene transferred by administering into the tail vein a bolus with ICL and MS. After 36 hours, body weight, plasma glucose, respiratory quotient and energy expenditure were measured. The respiratory quotient was increased by gene transfer, which suggests that a higher carbohydrate/lipid ratio is oxidized in such animals. This application could help, if adequate protocols are designed, to induce fat utilization of obesity and diabetes. [--]
Subject
Tail vein injection,
Plasmid delivery,
Liver,
Expression,
Rats,
UCP1,
Manipulation,
Mechanism,
Obesity,
Mice
Publisher
BioMed Central
Published in
Lipids in Health and Disease, 2008, 7: 49
Departament
Universidad Pública de Navarra. Departamento de Ciencias del Medio Natural /
Nafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Saila
Publisher version
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