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dc.creatorCordero, P.es_ES
dc.creatorCampión, J.es_ES
dc.creatorMilagro, F. I.es_ES
dc.creatorMarzo Pérez, Florencioes_ES
dc.creatorMartínez, J. A.es_ES
dc.date.accessioned2014-06-10T06:51:30Z
dc.date.available2014-06-10T06:51:30Z
dc.date.issued2008
dc.identifier.issn1476-511X
dc.identifier.other717
dc.identifier.urihttps://hdl.handle.net/2454/10790
dc.description.abstractThe glyoxylate cycle, which is well characterized in higher plants and some microorganisms but not in vertebrates, is able to bypass the citric acid cycle to achieve fat-to-carbohydrate interconversion. In this context, the hydrodynamic transfer of two glyoxylate cycle enzymes, such as isocytrate lyase (ICL) and malate synthase (MS), could accomplish the shift of using fat for the synthesis of glucose. Therefore, 20 mice weighing 23.37 +/- 0.96 g were hydrodinamically gene transferred by administering into the tail vein a bolus with ICL and MS. After 36 hours, body weight, plasma glucose, respiratory quotient and energy expenditure were measured. The respiratory quotient was increased by gene transfer, which suggests that a higher carbohydrate/lipid ratio is oxidized in such animals. This application could help, if adequate protocols are designed, to induce fat utilization of obesity and diabetes.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherBioMed Centralen
dc.relation.ispartofLipids in Health and Disease, 2008, 7: 49en
dc.rights© 2008 Cordero et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.subjectTail vein injectionen
dc.subjectPlasmid deliveryen
dc.subjectLiveren
dc.subjectExpressionen
dc.subjectRatsen
dc.subjectUCP1en
dc.subjectManipulationen
dc.subjectMechanismen
dc.subjectObesityen
dc.subjectMiceen
dc.titleFat-to-glucose interconversion by hydrodynamic transfer of two glyoxylate cycle enzyme genesen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentUniversidad Pública de Navarra. Departamento de Ciencias del Medio Naturales_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Sailaeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1186/1476-511X-7-49
dc.relation.publisherversionhttps://dx.doi.org/10.1186/1476-511X-7-49
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen


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© 2008 Cordero et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as © 2008 Cordero et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.