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dc.creatorEydallin, Gustavoes_ES
dc.creatorMontero Macarro, Manueles_ES
dc.creatorAlmagro Zabalza, Goizederes_ES
dc.creatorSesma Pascual, María Teresaes_ES
dc.creatorViale, Alejandro M.es_ES
dc.creatorMuñoz, Francisco Josées_ES
dc.creatorRahimpour, Mehdies_ES
dc.creatorBaroja Fernández, Edurnees_ES
dc.creatorPozueta Romero, Javieres_ES
dc.date.accessioned2014-06-11T09:17:59Z
dc.date.available2014-06-11T09:17:59Z
dc.date.issued2010
dc.identifier.issn1340-2838
dc.identifier.other646
dc.identifier.urihttps://hdl.handle.net/2454/10807
dc.description.abstractUsing a systematic and comprehensive gene expression library (the ASKA library), we have carried out a genome-wide screening of the genes whose increased plasmid-directed expression affected glycogen metabolism in Escherichia coli. Of the 4123 clones of the collection, 28 displayed a glycogen-excess phenotype, whereas 58 displayed a glycogen-deficient phenotype. The genes whose enhanced expression affected glycogen accumulation were classified into various functional categories including carbon sensing, transport and metabolism, general stress and stringent responses, factors determining intercellular communication, aggregative and social behaviour, nitrogen metabolism and energy status. Noteworthy, one-third of them were genes about which little or nothing is known. We propose an integrated metabolic model wherein E. coli glycogen metabolism is highly interconnected with a wide variety of cellular processes and is tightly adjusted to the nutritional and energetic status of the cell. Furthermore, we provide clues about possible biological roles of genes of still unknown functions.en
dc.description.sponsorshipThis research was partially supported by the grant BIO2007-63915 from the Comisión Interministerial de Ciencia y Tecnología and Fondo Europeo de Desarrollo Regional (Spain) and by Iden Biotechnology S.L.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherOxford University Pressen
dc.relation.ispartofDNA Research, 2010, 17(2). Págs. 61-71en
dc.rights© The Author 2010. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by-nc/2.5
dc.subjectASKA libraryen
dc.subjectCarbohydrate metabolismen
dc.subjectFunctional genomicsen
dc.subjectADP glucose pyrophosphorylaseen
dc.subjectAdenylate energy chargeen
dc.subjectBacterialen
dc.subjectGlycogenen
dc.subjectPhosphotransferase systemen
dc.subjectTyrosine phosphorylationen
dc.subjectProtein phosphorylationen
dc.subjectMulticellular behavioren
dc.subjectStringent responseen
dc.subjectCarbon metabolismen
dc.subjectBiofilm formationen
dc.titleGenome-wide screening of genes whose enhanced expression affects glycogen accumulation in Escherichia colien
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.affiliationNafarroako Unibertsitate Publikoa. Agrobioteknologiako Institutuaeu
dc.contributor.affiliationUniversidad Pública de Navarra. Instituto de Agrobiotecnologíaes_ES
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1093/dnares/dsp028
dc.relation.publisherversionhttps://dx.doi.org/10.1093/dnares/dsp028
dc.type.versionVersión aceptada / Onetsi den bertsioaes
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen


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© The Author 2010. Published by Oxford University Press on behalf of Kazusa DNA Research Institute.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium,
provided the original work is properly cited.
Except where otherwise noted, this item's license is described as © The Author 2010. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.