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dc.creatorRamírez, Hugoes_ES
dc.creatorReina Arias, Ramséses_ES
dc.creatorBertolotti, Luigies_ES
dc.creatorCenoz, Amaiaes_ES
dc.creatorHernández, Mirna Margaritaes_ES
dc.creatorSan Román Aberasturi, Beatrizes_ES
dc.creatorGlaría Ezquer, Idoiaes_ES
dc.creatorAndrés, Ximena dees_ES
dc.creatorCrespo, Helenaes_ES
dc.creatorJauregui, Paulaes_ES
dc.creatorBenavides, Julioes_ES
dc.creatorPolledo, Lauraes_ES
dc.creatorPérez, Valentínes_ES
dc.creatorGarcía Marín, Juan F.es_ES
dc.creatorRosati, Sergioes_ES
dc.creatorAmorena Zabalza, Beatrizes_ES
dc.creatorAndrés Cara, Damián dees_ES
dc.date.accessioned2014-06-13T11:37:38Z
dc.date.available2014-06-13T11:37:38Z
dc.date.issued2012
dc.identifier.issn1746-6148
dc.identifier.other537
dc.identifier.urihttps://hdl.handle.net/2454/10864
dc.description.abstractBackground: A central nervous system (CNS) disease outbreak caused by small ruminant lentiviruses (SRLV) has triggered interest in Spain due to the rapid onset of clinical signs and relevant production losses. In a previous study on this outbreak, the role of LTR in tropism was unclear and env encoded sequences, likely involved in tropism, were not investigated. This study aimed to analyze heterogeneity of SRLV Env regions - TM amino terminal and SU V4, C4 and V5 segments - in order to assess virus compartmentalization in CNS. Results: Eight Visna (neurologically) affected sheep of the outbreak were used. Of the 350 clones obtained after PCR amplification, 142 corresponded to CNS samples (spinal cord and choroid plexus) and the remaining to mammary gland, blood cells, bronchoalveolar lavage cells and/or lung. The diversity of the env sequences from CNS was 11.1-16.1% between animals and 0.35-11.6% within each animal, except in one animal presenting two sequence types (30% diversity) in the CNS (one grouping with those of the outbreak), indicative of CNS virus sequence heterogeneity. Outbreak sequences were of genotype A, clustering per animal and compartmentalizing in the animal tissues. No CNS specific signature patterns were found. Conclusions: Bayesian approach inferences suggested that proviruses from broncoalveolar lavage cells and peripheral blood mononuclear cells represented the common ancestors (infecting viruses) in the animal and that neuroinvasion in the outbreak involved microevolution after initial infection with an A-type strain. This study demonstrates virus compartmentalization in the CNS and other body tissues in sheep presenting the neurological form of SRLV infection.en
dc.description.sponsorshipThis study was supported by grants from Spanish CICYT (AGL2007-66874-C04, AGL2010-22341-C04) and Government of Navarra Department of Agriculture (IIQ010449.RI1 and IIQ14064.RI1). H. Ramirez was supported by PASPA program, UNAM and Public University of Navarra.en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherBioMed Centralen
dc.relation.ispartofBCM Veterinary Research, 2012, 8: 8en
dc.rights© 2012 Ramirez et al; BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.subjectCompartmentalizationen
dc.subjectVisnaen
dc.subjectSmall ruminant lentivirusen
dc.subjectSpinal corden
dc.subjectChoroid plexusen
dc.subjectSheepen
dc.subjectImmunodeficiency virus type 1en
dc.subjectCentral nervous systemen
dc.subjectFemaleen
dc.subjectGenital tracten
dc.subjectMaedi Visnaen
dc.subjectCerebroespinal fluiden
dc.subjectAntiretroviral therapyen
dc.subjectEnvelope glycoproteinen
dc.subjectResistance mutationsen
dc.subjectViral sequencesen
dc.subjectBlooden
dc.titleStudy of compartmentalization in the visna clinical form of small ruminant lentivirus infection in sheepen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentIdAB - Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1186/1746-6148-8-8
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/5PN/AGL2007-66874en
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/AGL2010-22341en
dc.relation.publisherversionhttps://dx.doi.org/10.1186/1746-6148-8-8
dc.type.versionVersión publicada / Argitaratu den bertsioaes
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua: IIQ010449.RI1
dc.contributor.funderGobierno de Navarra / Nafarroako Gobernua: IIQ14064.RI1
dc.contributor.funderUniversidad Pública de Navarra / Nafarroako Unibertsitate Publikoa


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© 2012 Ramirez et al; BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Except where otherwise noted, this item's license is described as © 2012 Ramirez et al; BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.