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dc.creatorSoler Lloréns, Pedroes_ES
dc.creatorGil Ramírez, Yolandaes_ES
dc.creatorZabalza Baranguá, Anaes_ES
dc.creatorIriarte, Maitees_ES
dc.creatorConde Álvarez, Raqueles_ES
dc.creatorZúñiga Ripa, Amaiaes_ES
dc.creatorSan Román Aberasturi, Beatrizes_ES
dc.creatorZygmunt, Micheles_ES
dc.creatorVizcaíno, Nieveses_ES
dc.creatorCloeckaert, Axeles_ES
dc.creatorGrilló Dolset, María Jesúses_ES
dc.creatorMoriyón Uría, Ignacioes_ES
dc.creatorLópez Goñi, Ignacioes_ES
dc.date.accessioned2015-10-27T08:36:36Z
dc.date.available2015-10-27T08:36:36Z
dc.date.issued2014
dc.identifier.issn0928-4249 (print)
dc.identifier.issn1297-9716 (electronic)
dc.identifier.urihttps://hdl.handle.net/2454/18635
dc.descriptionIncluye 1 fichero de datoses_ES
dc.description.abstractBrucella spp. are Gram-negative bacteria that behave as facultative intracellular parasites of a variety of mammals. This genus includes smooth (S) and rough (R) species that carry S and R lipopolysaccharides (LPS), respectively. S-LPS is a virulence factor, and mutants affected in the S-LPS O-polysaccharide (R mutants), core oligosaccharide or both show attenuation. However, B. ovis is naturally R and is virulent in sheep. We studied the role of B. ovis LPS in virulence by mutating the orthologues of wadA, wadB and wadC, three genes known to encode LPS core glycosyltransferases in S brucellae. When mapped with antibodies to outer membrane proteins (Omps) and R-LPS, wadB and wadC mutants displayed defects in LPS structure and outer membrane topology but inactivation of wadA had little or no effect. Consistent with these observations, the wadB and wadC but not the wadA mutants were attenuated in mice. When tested as vaccines, the wadB and wadC mutants protected mice against B. ovis challenge. The results demonstrate that the LPS core is a structure essential for survival in vivo not only of S brucellae but also of a naturally R Brucella pathogenic species, and they confirm our previous hypothesis that the Brucella LPS core is a target for vaccine development. Since vaccine B. melitensis Rev 1 is S and thus interferes in serological testing for S brucellae, wadB mutant represents a candidate vaccine to be evaluated against B. ovis infection of sheep suitable for areas free of B. melitensis.en
dc.description.sponsorshipThis work was funded by Ministerio de Economía y Competitividad of Spain (reference project AGL2011-30453-C04) and Fundación para la Investigación Médica Aplicada (FIMA). Financial support to PS-L from Ministerio de Economía y Competitividad of Spain (reference BES-2009-015656), AZ-B from Universidad Pública de Navarra, and BSR from CSIC and European Social Fund (Programa JAE-Doc) are also gratefully acknowledged.en
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherBioMed Centralen
dc.relation.ispartofVeterinary Research 2014, 45:72en
dc.rights© 2014 Soler-Lloréns et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en
dc.rights.urihttp://creativecommons.org/licenses/by/2.0
dc.rights.urihttp://creativecommons.org/publicdomain/zero/1.0/
dc.subjectLinked immunosorbent assayen
dc.subjectOuter membrane proteinen
dc.subjectMonoclonal-antibodiesen
dc.subjectO-polysaccharideen
dc.subjectRough lipopolysaccharideen
dc.subjectOMP25/OMP31 familyen
dc.subjectDNA polymorphismen
dc.subjectHost-rangeen
dc.subjectMelitensisen
dc.subjectVaccinesen
dc.titleMutants in the lipopolysaccharide of Brucella ovis are attenuated and protect against B. ovis infection in miceen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentUniversidad Pública de Navarra. Instituto de Agrobiotecnologíaes_ES
dc.contributor.departmentNafarroako Unibertsitate Publikoa. Agrobioteknologiako Institutuaeu
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1186/s13567-014-0072-0
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/AGL2011-30453
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/6PN/AGL2008-04514
dc.relation.publisherversionhttps://dx.doi.org/10.1186/s13567-014-0072-0
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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© 2014 Soler-Lloréns et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
La licencia del ítem se describe como © 2014 Soler-Lloréns et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.