Base pairing interaction between 5′- and 3′-UTRs controls icaR mRNA translation in Staphylococcus aureus
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Artículo / Artikulua
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The presence of regulatory sequences in the 39 untranslated region (39-UTR) of eukaryotic mRNAs controlling RNA stability and translation efficiency is widely recognized. In contrast, the relevance of 39-UTRs in bacterial mRNA functionality has been disregarded. Here, we report evidences showing that around one-third of the mapped mRNAs of the major human pathogen Staphylococcus aureus carry 3 ... [++]
The presence of regulatory sequences in the 39 untranslated region (39-UTR) of eukaryotic mRNAs controlling RNA stability and translation efficiency is widely recognized. In contrast, the relevance of 39-UTRs in bacterial mRNA functionality has been disregarded. Here, we report evidences showing that around one-third of the mapped mRNAs of the major human pathogen Staphylococcus aureus carry 39-UTRs longer than 100-nt and thus, potential regulatory functions. We selected the long 39-UTR of icaR, which codes for the repressor of the main exopolysaccharidic compound of the S. aureus biofilm matrix, to evaluate the role that 39-UTRs may play in controlling mRNA expression. We showed that base pairing between the 39- UTR and the Shine-Dalgarno (SD) region of icaR mRNA interferes with the translation initiation complex and generates a double-stranded substrate for RNase III. Deletion or substitution of the motif (UCCCCUG) within icaR 39-UTR was sufficient to abolish this interaction and resulted in the accumulation of IcaR repressor and inhibition of biofilm development. Our findings provide a singular example of a new potential post-transcriptional regulatory mechanism to modulate bacterial gene expression through the interaction of a 39-UTR with the 59-UTR of the same mRNA. [--]
Base pairing interaction, Staphylococcus aureus, mRNA expression, 5-UTR, 3-UTR, icaR
Public Library of Science
PLoS Genetics 9(12): e1004001
UPNa. Instituto de Agrobiotecnología. Laboratorio de Biofilms Microbianos.Incluye 10 ficheros de datos
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
This work was supported by grants from Spanish Ministry of Economy and Competitiveness (BFU2011-23222, BIO2008-05284-C02-01 and ERA-NET Pathogenomics PIM2010EPA-00606) and from Fundação para a Ciência e Tecnologia - Portugal (ERA-PTG/0002/2010 and PEst-OE/EQB/LA0004/2011). IRdlM and JV were supported by F.P.I. (BES-2009-017410) and Ramón y Cajal (RYC-2009-03948) contracts, respectively, from the Spanish Ministry of Economy and Competitiveness.
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