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    Coordinated cyclic-di-GMP repression of salmonella motility through YcgR and cellulose

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    Fecha
    2013
    Autor
    Zorraquino Salvo, Violeta 
    García Martínez, Begoña Upna
    Latasa Osta, Cristina Upna
    Echeverz Sarasúa, Maite Upna
    Toledo Arana, Alejandro Upna
    Valle Turrillas, Jaione Upna
    Lasa Uzcudun, Íñigo Upna
    Solano Goñi, Cristina Upna
    Versión
    Acceso abierto / Sarbide irekia
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    Artículo / Artikulua
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    Versión aceptada / Onetsi den bertsioa
    Impacto
     
     
     
    10.1128/JB.01789-12
     
     
     
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    Resumen
    Cyclic di-GMP (c-di-GMP) is a secondary messenger that controls a variety of cellular processes, including the switch between a biofilm and a planktonic bacterial lifestyle. This nucleotide binds to cellular effectors in order to exert its regulatory functions. In Salmonella, two proteins, BcsA and YcgR, both of them containing a c-di-GMP binding PilZ domain, are the only known c-di-GMP receptors ... [++]
    Cyclic di-GMP (c-di-GMP) is a secondary messenger that controls a variety of cellular processes, including the switch between a biofilm and a planktonic bacterial lifestyle. This nucleotide binds to cellular effectors in order to exert its regulatory functions. In Salmonella, two proteins, BcsA and YcgR, both of them containing a c-di-GMP binding PilZ domain, are the only known c-di-GMP receptors. BcsA, upon c-di-GMP binding, synthesizes cellulose, the main exopolysaccharide of the biofilm matrix. YcgR is dedicated to c-di-GMP-dependent inhibition of motility through its interaction with flagellar motor proteins. However, previous evidences indicate that in the absence of YcgR, there is still an additional element that mediates motility impairment under high c-di-GMP levels. Here we have uncovered that cellulose per se is the factor that further promotes inhibition of bacterial motility once high c-di-GMP contents drive the activation of a sessile lifestyle. Inactivation of different genes of the bcsABZC operon, mutation of the conserved residues in the RxxxR motif of the BcsA PilZ domain, or degradation of the cellulose produced by BcsA rescued the motility defect of ΔycgR strains in which high c-di-GMP levels were reached through the overexpression of diguanylate cyclases. High c-di-GMP levels provoked cellulose accumulation around cells that impeded flagellar rotation, probably by means of steric hindrance, without affecting flagellum gene expression, exportation, or assembly. Our results highlight the relevance of cellulose in Salmonella lifestyle switching as an architectural element that is both essential for biofilm development and required, in collaboration with YcgR, for complete motility inhibition. [--]
    Materias
    Cyclic di-GMP, Salmonella, Cellulose, YcgR, Motility, Biofilms
     
    Editor
    American Society for Microbiology
    Publicado en
    Journal of Bacteriology, February 2013, vol. 195, no. 3, 417-428
    Notas
    Incluye 1 fichero de datos
    Departamento
    Universidad Pública de Navarra. Instituto de Agrobiotecnología / Nafarroako Unibertsitate Publikoa. Agrobioteknologiako Institutua
     
    Versión del editor
    https://dx.doi.org/10.1128/JB.01789-12
    URI
    https://hdl.handle.net/2454/22577
    Entidades Financiadoras
    J.V. was supported by a Ramón y Cajal contract from the Ministerio de Economía y Competitividad, Spain. A JAE predoctoral research contract for V.Z. from the Consejo Superior de Investigaciones Científicas (CSIC, Spain) is gratefully acknowledged. This research was supported by a grant from the Departamento de Salud (Resolución 1312/2010), Gobierno de Navarra, Spain.
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