Genome-wide antisense transcription drives mRNA processing in bacteria
Fecha
2011Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión aceptada / Onetsi den bertsioa
Impacto
|
10.1073/pnas.1113521108
Resumen
RNA deep sequencing technologies are revealing unexpected levels of complexity in bacterial transcriptomes with the discovery of abundant noncoding RNAs, antisense RNAs, long 5′ and 3′ untranslated regions, and alternative operon structures. Here, by applying deep RNA sequencing to both the long and short RNA fractions (<50 nucleotides) obtained from the major human pathogen Staphylococcus aureus ...
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RNA deep sequencing technologies are revealing unexpected levels of complexity in bacterial transcriptomes with the discovery of abundant noncoding RNAs, antisense RNAs, long 5′ and 3′ untranslated regions, and alternative operon structures. Here, by applying deep RNA sequencing to both the long and short RNA fractions (<50 nucleotides) obtained from the major human pathogen Staphylococcus aureus, we have detected a collection of short RNAs that is generated genome-wide through the digestion of overlapping sense/antisense transcripts by RNase III endoribonuclease. At least 75% of sense RNAs from annotated genes are subject to this mechanism of antisense processing. Removal of RNase III activity reduces the amount of short RNAs and is accompanied by the accumulation of discrete antisense transcripts. These results suggest the production of pervasive but hidden antisense transcription used to process sense transcripts by means of creating double-stranded substrates. This process of RNase III-mediated digestion of overlapping transcripts can be observed in several evolutionarily diverse Gram-positive bacteria and is capable of providing a unique genome-wide posttranscriptional mechanism to adjust mRNA levels. [--]
Materias
Antisense RNA,
Overlapping transcription,
RNA processing,
Posttranscriptional regulation,
MicroRNA
Editor
National Academy of Sciences
Publicado en
PNAS, December 13, 2011 vol. 108 no. 50
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
I.L. was
supported by a “Salvador Madariaga” fellowship from the Spanish Ministry
of Science and Innovation. A.T.-A. and J.V. were supported by Spanish Ministry
of Science and Innovation “Ramon y Cajal” contracts. M.V. was supported
by a Consejo Superior de Investigaciones Científicas JAE Predoctoral
research contract. This work was supported by Spanish Ministry of Science
and Innovation Grants BIO2008-05284-C02-01 and ERA-NET Pathogenomics
PIM2010EPA-00606.