Staphylococcal Bap proteins build amyloid scaffold biofilm matrices in response to environmental signals
Fecha
2016Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Identificador del proyecto
Impacto
|
10.1371/journal.ppat.1005711
Resumen
Major components of the biofilm matrix scaffold are proteins that assemble to create a unified structure that maintain bacteria attached to each other and to surfaces. We provide evidence that a surface protein present in several staphylococcal species forms functional amyloid aggregates to build the biofilm matrix in response to specific environmental conditions. Under low Ca2+ concentrations an ...
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Major components of the biofilm matrix scaffold are proteins that assemble to create a unified structure that maintain bacteria attached to each other and to surfaces. We provide evidence that a surface protein present in several staphylococcal species forms functional amyloid aggregates to build the biofilm matrix in response to specific environmental conditions. Under low Ca2+ concentrations and acidic pH, Bap is processed and forms insoluble aggregates with amyloidogenic properties. When the Ca2+ concentration increases, metal-coordinated Bap adopts a structurally more stable conformation and as a consequence, the N-terminal region is unable to assemble into amyloid aggregates. The control of Bap cleavage and assembly helps to regulate biofilm matrix development as a function of environmental changes. [--]
Materias
Staphylococcus aureus,
Bacterial biofilms,
Amyloid proteins,
Extracellular matrix
Editor
Public Library of Science
Publicado en
PLoS Pathogens 12(6): e1005711
Departamento
Universidad Pública de Navarra/Nafarroako Unibertsitate Publikoa. IdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutua
Versión del editor
Entidades Financiadoras
This research was supported by the
Spanish Ministry of Economy and Competitiveness
grants AGL2011-23954, BIO2014-53530-R and
BFU2013-44763-P. JV was supported by Ramon y
Cajal (RYC-2009-03948) contract from the Spanish
Ministry of Economy and Competitiveness.