Characterization of the Amaranthus palmeri physiological response to glyphosate in susceptible and resistant populations
Date
2016Author
Version
Acceso abierto / Sarbide irekia
Type
Artículo / Artikulua
Version
Versión aceptada / Onetsi den bertsioa
Impact
|
10.1021/acs.jafc.5b04916
Abstract
The herbicide glyphosate inhibits the plant enzyme 5-enolpyruvylshikimate3-phosphate synthase (EPSPS) in the aromatic amino acid (AAA) biosynthetic pathway. The physiologies of an Amaranthus palmeri population exhibiting resistance to glyphosate by EPSPS gene amplification (NC-R) and a susceptible population (NC-S) were compared. The EPSPS copy number of NC-R plants was 47.5-fold the copy number ...
[++]
The herbicide glyphosate inhibits the plant enzyme 5-enolpyruvylshikimate3-phosphate synthase (EPSPS) in the aromatic amino acid (AAA) biosynthetic pathway. The physiologies of an Amaranthus palmeri population exhibiting resistance to glyphosate by EPSPS gene amplification (NC-R) and a susceptible population (NC-S) were compared. The EPSPS copy number of NC-R plants was 47.5-fold the copy number of NC-S plants. Although the amounts of EPSPS protein and activity were higher in NC-R plants than in NC-S plants, the AAA concentrations were similar. The increases in total free amino acid and in AAA contents induced by glyphosate were more evident in NC-S plants. In both populations, the EPSPS protein increased after glyphosate exposure, suggesting regulation of gene expression. EPSPS activity seems tightly controlled in vivo. Carbohydrate accumulation and a slight induction of ethanol fermentation were detected in both populations. [--]
Subject
Carbohydrate accumulation,
Ethanol fermentation,
Free amino acid accumulation,
Herbicide resistance,
Physiological effects
Publisher
ACS Publications
Published in
Journal of Agricultural and Food Chemistry, 2016, 64 (1), pp 95–106
Description
Incluye 2 ficheros de datos
Departament
Universidad Pública de Navarra. Departamento de Ciencias del Medio Natural /
Nafarroako Unibertsitate Publikoa. Natura Ingurunearen Zientziak Saila
Publisher version
Sponsorship
This work was financially supported by a grant from the
Ministerio Español de Economıa y Competitividad (AGL-
2013-40567R). M.F.-E. and M.G.-M. received funding from fellowships through the Universidad Publica de Navarra.