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dc.creatorCaballo Ponce, Eloyes_ES
dc.creatorMurillo Martínez, Jesúses_ES
dc.creatorMartínez Gil, Martaes_ES
dc.creatorMoreno Pérez, Albaes_ES
dc.creatorPintado, Adriánes_ES
dc.creatorRamos, Cayoes_ES
dc.identifier.issn1664-462X (Electronic)
dc.description.abstractThe study of the molecular basis of tree diseases is lately receiving a renewed attention, especially with the emerging perception that pathogens require specific pathogenicity and virulence factors to successfully colonize woody hosts. Pathosystems involving woody plants are notoriously difficult to study, although the use of model bacterial strains together with genetically homogeneous micropropagated plant material is providing a significant impetus to our understanding of the molecular determinants leading to disease. The gammaproteobacterium Pseudomonas savastanoi belongs to the intensively studied Pseudomonas syringae complex, and includes three pathogenic lineages causing tumorous overgrowths (knots) in diverse economically relevant trees and shrubs. As it occurs with many other bacteria, pathogenicity of P. savastanoi is dependent on a type III secretion system, which is accompanied by a core set of at least 20 effector genes shared among strains isolated from olive, oleander, and ash. The induction of knots of wild-type size requires that the pathogen maintains adequate levels of diverse metabolites, including the phytohormones indole-3-acetic acid and cytokinins, as well as cyclic-di-GMP, some of which can also regulate the expression of other pathogenicity and virulence genes and participate in bacterial competitiveness. In a remarkable example of social networking, quorum sensing molecules allow for the communication among P. savastanoi and other members of the knot microbiome, while at the same time are essential for tumor formation. Additionally, a distinguishing feature of bacteria from the P. syringae complex isolated from woody organs is the possession of a 15 kb genomic island (WHOP) carrying four operons and three other genes involved in degradation of phenolic compounds. Two of these operons mediate the catabolism of anthranilate and catechol and, together with another operon, are required for the induction of full-size tumors in woody hosts, but not in non-woody micropropagated plants. The use of transposon mutagenesis also uncovered a treasure trove of additional P. savastanoi genes affecting virulence and participating in diverse bacterial processes. Although there is still much to be learned on what makes a bacterium a successful pathogen of trees, we are already untying the knots.en
dc.description.sponsorshipThis work was supported by the Spanish Plan Nacional I+D+i grants AGL2014-53242-C2-1-R and AGL2014-53242-C2-2-R from the Spanish Ministerio de Economía y Competitividad (MINECO) and was co-financed by FEDER. AP was supported by a FPU Ph.D. fellowship and AM-P and EC-P by FPI Ph.D. fellowships from the Spanish Ministerio de Educación, Cultura y Deporte (MECD) and MINECO.en
dc.publisherFrontiers Mediaen
dc.relation.ispartofFrontiers in Plant Science 8:1089en
dc.rightsCreative Commons Attribution 4.0 International (CC BY 4.0).en
dc.subjectBacterial gallen
dc.subjectOlive knoten
dc.subjectWoody hosten
dc.subjectPseudomonas savastanoien
dc.subjectPseudomonas syringaeen
dc.titleKnots untie: molecular determinants involved in knot formation Induced by Pseudomonas savastanoi in woody hostsen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentProducción Agrariaes_ES
dc.contributor.departmentNekazaritza Ekoizpenaeu
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.type.versionVersión publicada / Argitaratu den bertsioaes

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Creative Commons Attribution 4.0 International (CC BY 4.0).
Except where otherwise noted, this item's license is described as Creative Commons Attribution 4.0 International (CC BY 4.0).

El Repositorio ha recibido la ayuda de la Fundación Española para la Ciencia y la Tecnología para la realización de actividades en el ámbito del fomento de la investigación científica de excelencia, en la Línea 2. Repositorios institucionales (convocatoria 2020-2021).
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