In situ RNA-RNA hybridization: a useful method for analysis of the distribution of transcripts of various genes in Lentinula edodes fruiting bodies
Fecha
2006Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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nodoi-noplumx
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Resumen
The in situ RNA-RNA hybridization was renewed understanding that it is a useful method for analysis
of the distribution of transcripts of various genes in fruiting bodies of Lentinula edodes. By using
this method, we obtained the following results. Large amounts of the transcripts of ribonucleotide reductase
small subunit gene (Le.rnr2) and UMP-CMP kinase gene (uck1), which is a target of deve ...
[++]
The in situ RNA-RNA hybridization was renewed understanding that it is a useful method for analysis
of the distribution of transcripts of various genes in fruiting bodies of Lentinula edodes. By using
this method, we obtained the following results. Large amounts of the transcripts of ribonucleotide reductase
small subunit gene (Le.rnr2) and UMP-CMP kinase gene (uck1), which is a target of developmental
regulator PRIB, are present in both hymenium and outer region of trama in the hymenophore
(gill tissue). The hymenium is the part for production of basidiospores and the outer
region of trama is the region branching out into subhymenium (on the top of which hymenium is
formed). The Le.ras transcript is present mostly in outer region of trama and in trama cells, while the
transcript of trimeric G-protein αsubunit gene (Le.ga1) is mostly in hymenium. The transcript of
mfbC gene, which is the target of PRIB and probably encodes the protein interacting with a putative
translation initiation factor 5A (eIF5A), is present in outer region of trama. The transcript of hyd1
(hydrophobin 1 gene), whose expressed product is considered to be involved in the formation in the
extracellular matrix of lined air channels with a hydrophobic membrane, is present everywhere in the
mycelial tissues of developing fruiting bodies except for the top parts of pileus (cap) and for prehymenophore.
The region surrounding prehymenophore contains a high level of the transcript. These
results suggest that Le.rnr2 and uck1 genes play a role mainly in the nucleotide biosynthesis essential
for production of basidiospores and for divergence of trama cells into subhymenium cells. The Le.ras
and mfbC play a role in divergence of mycelial cells and the Le.ga1 plays a role in spore-production.
The hydrophobin-mediated air channels may be formed almost all the parts of developing fruiting
bodies. [--]
Materias
In situ RNA-RNA hybridization,
Lentinula edodes,
Gene transcription
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Comunicación presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
Entidades Financiadoras
This work was supported by a Grant-in Aid for Scientific Research (no.
13660084) from the Ministry of Education, Culture, Sports, Science, and
Technology of Japan.