Overexpression of laccases in Coprinopsis cinerea
Fecha
2006Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Laccases are versatile redox-enzymes that oxidize various phenolic compounds and aromatic amines.
Because of the broad substrate specificity, these enzymes are of interest for many different biotechnological
applications. White-rot fungi are the major source of laccases. Although basidiomycete species
give rise to relatively high enzyme yields, these might not be optimal for applications. Basi ...
[++]
Laccases are versatile redox-enzymes that oxidize various phenolic compounds and aromatic amines.
Because of the broad substrate specificity, these enzymes are of interest for many different biotechnological
applications. White-rot fungi are the major source of laccases. Although basidiomycete species
give rise to relatively high enzyme yields, these might not be optimal for applications. Basidiomycetous
laccases have been reported to show hyper- or hypo-glycosylation when expressed in ascomycetes.
In consequence, enzyme characteristics were found to be altered. Therefore, we use the basidiomycete
Coprinopsis cinerea as an organism for laccase overproduction. We present a vector system for easy and
rapid cloning of promoters and/or genes of interest and show that such constructs can be functional in
laccase production. [--]
Materias
Lacasses,
Coprinopsis cinerea
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Comunicación presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
Entidades Financiadoras
The author's laboratory is funded by the DBU (Deutsche Bundesstiftung
Umwelt). The authors are members of the NHN (Niedersächsisches
Kompetenznetz für Nachhaltige Holznutzung). Within the NHN, this work
is supported by the European Regional Development Fund (EFRE project
2001.085) and the BMVEL (FKZ 22010603, 2005).