Genetic analysis of Coprinopsis cinerea mutants with defects in fruiting body development
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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nodoi-noplumx
|
Resumen
Few genes have so far been cloned and characterized in fruiting of the heterothallic mushroom Coprinopsis
cinerea. Fruiting body development normally occurs on the dikaryon. However, the binucleate
state of the mycelium hinders easy access of genes. Self-compatible mutants with defects in the
mating type pathways can form fruiting bodies without prior fusion to another strain. Uninucleate
hap ...
[++]
Few genes have so far been cloned and characterized in fruiting of the heterothallic mushroom Coprinopsis
cinerea. Fruiting body development normally occurs on the dikaryon. However, the binucleate
state of the mycelium hinders easy access of genes. Self-compatible mutants with defects in the
mating type pathways can form fruiting bodies without prior fusion to another strain. Uninucleate
haploid oidia of such mutants can easily be mutagenized and germinated mycelia tested for defects in
fruiting. Mutants can be produced from oidia by classical techniques such as UV treatment or by modern
REMI (restriction enzyme-mediated integration) mutagenesis via transformation. Such mutants
of self-compatible strains have now been successfully appointed in cloning genes acting in sexual development.
Co-isogenic strains of compatible mating types support in genetic characterisation of the
mutants. [--]
Materias
Coprinopsis cinerea,
Fruiting body development,
Mutants
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Comunicación presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
Entidades Financiadoras
PS and WC hold PhD scholarships of the Mahasarakham University (Thailand)
and the University of Technology Lanna Phitsanulok Campus (Thailand),
respectively. The author's laboratory is supported by the Deutsche Bundesstiftung
Umwelt (DBU).