Gene expression during symbiosis in the ectomycorrhizal fungus Tricholoma vaccinum
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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nodoi-noplumx
|
Resumen
We have identified genes specifically expressed in host interaction using an
RNA fingerprinting technique. These genes can subsequently be used to
identify host signals for induction of genes with function in host specificity.
The easy method allowed to screen RNA from mycorrhiza of different age (2
weeks to 13 months) synthesized in vitro in comparison to RNA from both
partners, Tricholoma ...
[++]
We have identified genes specifically expressed in host interaction using an
RNA fingerprinting technique. These genes can subsequently be used to
identify host signals for induction of genes with function in host specificity.
The easy method allowed to screen RNA from mycorrhiza of different age (2
weeks to 13 months) synthesized in vitro in comparison to RNA from both
partners, Tricholoma vaccinum and Picea abies, grown in single culture. The
resulting cDNA was used in an arbitrarily primed PCR using 5 primers
which had been selected out of 20 to give more than 20 bands of a size up to
1.9 kb. From 145 bands separated on agarose gels 52% were differentially expressed.
The fungal genes were identified by Southern blot analyses and subsequenly
differential expression was verifie by reversed Northern blots. Since
the bands were, in many cases, still containing more than a single fragment,
130 fragmetns were cloned, of which 57 were mykorrhiza-specific.
Of 23 fungal genes with mycorrhiza-specific expression, sequence analyses
were performed in order to identify the nature of the encoded protein in
silico. Among them different classes of function were defined with respect to
putative function of the respective gene in the symbiosis. Examples with possible
ecological function include aldehyde and alcohol dehydrogenases, ubiquitin
binding protein, phospholipase B, ß-1,4 glucosidase, a binding protein
for basic amino acids, an APS kinase, two MATE transporters and Ras. The
possible roles of these proteins will be discussed. Additionally, two different
classes of retrotransposon were identified which is the first identificaiton of
actively expressed trnasposons in ectomycorrhizal fungi which might be the
reason for high morphological diversity observed with T. vaccinum. [--]
Materias
Tricholoma vaccinum
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.