Biochemistry of volatile compounds synthesis in Agaricus bisporus
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Agaricus bisporus unique flavour is due to the release of a set of eight-carbon
volatile compounds, which biosynthetic pathway has not been elucidated yet,
despite of the numerous implications of those volatile compounds. Beside
their influence on crop quality, they are also important for insect perception
and play a part in triggering the switch from vegetative to reproductive
growth in mus ...
[++]
Agaricus bisporus unique flavour is due to the release of a set of eight-carbon
volatile compounds, which biosynthetic pathway has not been elucidated yet,
despite of the numerous implications of those volatile compounds. Beside
their influence on crop quality, they are also important for insect perception
and play a part in triggering the switch from vegetative to reproductive
growth in mushrooms.
8-carbon volatiles are derived from the oxygenation and the cleavage of
the polyunsaturated fatty acid linoleic acid. This reaction has similarities to
the plant system, but also major differences. Examination of the enzymic
mechanisms and the fatty acid chemistry suggested that the enzyme involved
in the oxygenation step could be a lipoxygenase (as found in plants) or a
heme-dioxygenase, similar to the recently isolated linoleate diol synthase
from Gaeumannomyces graminis.
In order to characterise the biochemical pathway leading to eight-carbon
volatile production, we investigated fatty acid and lipids distribution in Agaricus
bisporus, as well as hydroperoxide and volatile compounds levels. In parallel,
we searched for candidate genes susceptible to encode the enzyme responsible
for this novel oxidation route in fungi.
The combination of analytical methods, such as GC-MS, with a molecular
approach based on degenerate PCR and library screening provided us
with a broad range of results. These results establish the relation between fatty
acids and volatile compounds and enabled us to gain a better understanding
of mushroom volatiles biosynthesis and lipid metabolism. [--]
Materias
Agaricus bisporus,
Volatile compounds
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.