Virus related symptoms in crops of the button mushroom Agaricus bisporus
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Since more than five years symptoms are seen in Dutch mushroom crops
that correlate with the presence of a set of dsRNAs. These dsRNAs are indicative
for the presence of viruses. Symptoms are discoloration of white
mushrooms varying from cream to brown. Crops can be heavily affected with
more than 50% of the mushrooms showing discoloration. Mostly, however,
crops show mild symptoms with a few per ...
[++]
Since more than five years symptoms are seen in Dutch mushroom crops
that correlate with the presence of a set of dsRNAs. These dsRNAs are indicative
for the presence of viruses. Symptoms are discoloration of white
mushrooms varying from cream to brown. Crops can be heavily affected with
more than 50% of the mushrooms showing discoloration. Mostly, however,
crops show mild symptoms with a few percentages of mushrooms showing
an off-white color. Since traiders associate this discoloration with low quality
economical damage can be substantial for individual farmers.
In affected crops up to 15 dsRNAs are observed with varying intensity.
Only the five shortest dsRNAs show a perfect quantitative and qualitative
correlation with the symptom. They are always present in discolored mushrooms
and the intensity of the bands seen in agarose gels vary with the intensity
of the discoloration. Most of the other dsRNAs are also present in affected
crops but are also seen in crops without any visual symptoms. We have
started a molecular analysis of the dsRNAs in order to design sensitive tests
for each dsRNA. This will reveal if all or some dsRNAs are always present in
mushrooms or are derived from an unknown infectious source.
From a number of dsRNAs 60 to 80% of the sequence has been determined.
RT-PCR tests have been designed recently for these dsRNAs and the
first result of tests of several crops will be presented. [--]
Materias
Agaricus bisporus,
Double stranded RNA (dsRNA)
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.