Double stranded RNAs in the mushroom virus X complex of Agaricus bisporus
Fecha
2006Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Mushroom virus X (MVX) is a new disease of the commercial mushroom,
Agaricus bisporus. When first recorded in 1996 it was associated with localised
areas of pin suppression. It is now thought to be responsible for a range of
symptoms. It seriously affected the UK mushroom industry in 2000/01 and
has now been reported in Ireland, Holland and a number of other mushroom
growing countries. The o ...
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Mushroom virus X (MVX) is a new disease of the commercial mushroom,
Agaricus bisporus. When first recorded in 1996 it was associated with localised
areas of pin suppression. It is now thought to be responsible for a range of
symptoms. It seriously affected the UK mushroom industry in 2000/01 and
has now been reported in Ireland, Holland and a number of other mushroom
growing countries. The objectives of the work programme put in place to address
this problem were to determine the relationship between major dsRNA
bands present in the MVX complex and to sequence characterise major components;
to develop an RT-PCR diagnostic test for separate viruses within the
MVX complex and test commercial spawns and ARP cultures for virus presence;
to characterise transmission of dsRNA elements between strains, assess
effects of cross infection and determine significance of dsRNA partitioning
during transmission through spores; and to determine the rate of spread of
MVX into healthy compost from a point source
Analysis of diseased mushrooms has identified 26 double stranded RNA
(dsRNA) molecules associated with MVX symptoms. Evidence collected to
date suggests that MVX might be a complex of viruses, rather than a single
virus. Three of these dsRNAs have also been shown to be present in asymptomatic
mushrooms. Vertical transmission was shown to occur through production
of single spore progeny isolates from infected sporophores. Partitioning
of dsRNAs was shown to occur. To determine whether horizontal
transmission occurred, dual-culture in vitro tests were performed using
donor and acceptor strains. Cultures produced from these transmission lines
contained a reduced number of dsRNAs compared with the original donor
strains.
Sequence data has been generated for several dsRNAs. [--]
Materias
Mushroom virus X (MVX),
Agaricus bisporus,
Double stranded RNA (dsRNA)
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen de la conferencia presentada al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.