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    • Congresos y Publicaciones de la UPNA - NUPeko Biltzarrak eta Argitalpenak
    • Congresos UPNA - NUPeko Biltzarrak
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    • VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI)
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    The Coprinus cinereus genome project

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    PA01_Coprinus.pdf (61.57Kb)
    Date
    2006
    Author
    Gathman, A. 
    Lilly, W. 
    Stajich, J. 
    Carlson, M. 
    Murphy, B. 
    Smith, A. 
    Fargo, D. 
    Dietrich, F. 
    Pukkila, P.J. 
    Version
    Acceso abierto / Sarbide irekia
    xmlui.dri2xhtml.METS-1.0.item-type
    Contribución a congreso / Biltzarrerako ekarpena
    Version
    Versión publicada / Argitaratu den bertsioa
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    Abstract
    Coprinus cinereus is an increasingly attractive basidiomycete model system. Its genome has been sequenced and is publicly available; it is readily cultured in the laboratory on defined media, it has highly synchronous meiosis, and numerous laboratory techniques have been adapted for use with it. The 10X shotgun sequence released by the Whitehead Institute comprises 36 Mb of the 37 Mb of the genom ... [++]
    Coprinus cinereus is an increasingly attractive basidiomycete model system. Its genome has been sequenced and is publicly available; it is readily cultured in the laboratory on defined media, it has highly synchronous meiosis, and numerous laboratory techniques have been adapted for use with it. The 10X shotgun sequence released by the Whitehead Institute comprises 36 Mb of the 37 Mb of the genome, which have been assembled into 106 supercontigs containing 431 contigs. cDNA libraries have been constructed from two meiotic stages, and 1432 candidate genes have been identified from them. Another set of cDNA libraries has been constructed from vegetative Coprinus cinereus Okayama 7 grown under different environmental conditions, including heat shock, rapamycin treatment, minimal medium, rich medium, and complex carbon and nitrogen sources. 5000 ESTs are being sequenced from these libraries. The EST sequences have been aligned with the genomic sequence, as have known C. cinereus genes from GenBank. Data from known ascomycete gene sequences have been used to train SNAP software to predict a total of 11,340 genes from the remainder of the genome. BlastX and Pfam have been used to assign tentative functions to predicted genes as well as ESTs. tRNA genes have also been identified in the genome. All genomic information is available online via our Gbrowse server. [--]
    Subject
    Coprinus cinereus
    Publisher
    Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
    Published in
    Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
    Description
    Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
    URI
    https://hdl.handle.net/2454/27258
    Appears in Collections
    • VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI) [60]
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