The Coprinus cinereus genome project
Fecha
2006Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
|
nodoi-noplumx
|
Resumen
Coprinus cinereus is an increasingly attractive basidiomycete model system. Its
genome has been sequenced and is publicly available; it is readily cultured in
the laboratory on defined media, it has highly synchronous meiosis, and numerous
laboratory techniques have been adapted for use with it. The 10X
shotgun sequence released by the Whitehead Institute comprises 36 Mb of
the 37 Mb of the ...
[++]
Coprinus cinereus is an increasingly attractive basidiomycete model system. Its
genome has been sequenced and is publicly available; it is readily cultured in
the laboratory on defined media, it has highly synchronous meiosis, and numerous
laboratory techniques have been adapted for use with it. The 10X
shotgun sequence released by the Whitehead Institute comprises 36 Mb of
the 37 Mb of the genome, which have been assembled into 106 supercontigs
containing 431 contigs. cDNA libraries have been constructed from two
meiotic stages, and 1432 candidate genes have been identified from them.
Another set of cDNA libraries has been constructed from vegetative Coprinus
cinereus Okayama 7 grown under different environmental conditions, including
heat shock, rapamycin treatment, minimal medium, rich medium,
and complex carbon and nitrogen sources. 5000 ESTs are being sequenced
from these libraries. The EST sequences have been aligned with the genomic
sequence, as have known C. cinereus genes from GenBank. Data from
known ascomycete gene sequences have been used to train SNAP software
to predict a total of 11,340 genes from the remainder of the genome. BlastX
and Pfam have been used to assign tentative functions to predicted genes as
well as ESTs. tRNA genes have also been identified in the genome. All genomic
information is available online via our Gbrowse server. [--]
Materias
Coprinus cinereus
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.