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    • Congresos y Publicaciones de la UPNA - NUPeko Biltzarrak eta Argitalpenak
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    • VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI)
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    Species identification and detection of fungi in biological materials by FTIR microscopy

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    PA11_Species.pdf (53.77Kb)
    Date
    2006
    Author
    Naumann, Anette 
    Navarro González, Mónica 
    Peddireddi, Sudhakar 
    Schützendübel, Andres 
    Kües, Ursula 
    Polle, Andrea 
    Version
    Acceso abierto / Sarbide irekia
    xmlui.dri2xhtml.METS-1.0.item-type
    Contribución a congreso / Biltzarrerako ekarpena
    Version
    Versión publicada / Argitaratu den bertsioa
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    Abstract
    FTIR spectroscopy provides the opportunity to simultaneously detect many molecular bonds or functional groups of different polysaccharides, proteins, lipids, aromatic and other compounds. The measurement principle is based on the absorption of infrared light by dipolar molecular bonds. In combination with microscopy, local resolution of the chemical composition is possible. Each absorption p ... [++]
    FTIR spectroscopy provides the opportunity to simultaneously detect many molecular bonds or functional groups of different polysaccharides, proteins, lipids, aromatic and other compounds. The measurement principle is based on the absorption of infrared light by dipolar molecular bonds. In combination with microscopy, local resolution of the chemical composition is possible. Each absorption point or peak in the spectrum can be integrated to create an image of the distribution of the corresponding compound. We use FTIR-microscopy in order to detect fungi in plant tissues such as in infected wood and in mycorhizal roots. For the development of a fast and inexpensive method for localisation and identification of fungi, differences between FTIR measurements of fungi and plant cells are characterized. In addition, FTIR spectra of different fungi are compared. Beech wood blocks were infected with Trametes versicolor and with Schizophyllum commune and FTIR spectra in sections of the infected wood determined. Cluster analysis revealed major differences between FTIR spectra recorded from wood fibres and empty vessel lumina and spectra from fungal mycelium, irrespectively of whether grown on the surface of wood or inside vessel lumina. Species specific clustering of spectra of fungal mycelium grown on the wood surface and inside vessel lumina demonstrated the potential of FTIR microscopy to identify fungal mycelium in wood. Currently, we are sampling FTIR spectra from various basidiomycetes in order to define species according to their specific FTIR spectra. The work is supported in frame of the Lower Saxony Competence Network for Sustainable Timber Utilisation (NHN) by the Ministry of Culture of Lower Saxony and EFRE. The group of UK is funded by the DBU. MNG holds a PhD studentship from CONACYT, Mexico. [--]
    Subject
    FTIR microscopy, Species identification, Detection of fungi
     
    Publisher
    Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
    Published in
    Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
    Description
    Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
    URI
    https://hdl.handle.net/2454/27268
    Sponsorship
    The work is supported in frame of the Lower Saxony Competence Network for Sustainable Timber Utilisation (NHN) by the Ministry of Culture of Lower Saxony and EFRE. The group of UK is funded by the DBU. MNG holds a PhD studentship from CONACYT, Mexico.
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    • VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI) [60]
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