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    • VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI)
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    Computational prediction of protein-coding gene and annotation of DNA sequences with agronomic interest in Pleurotus ostreatus (Oyster Mushroom)

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    Date
    2006
    Author
    Palma Dovis, Leopoldo Upna Orcid
    Peñas Parrila, María Manuela 
    Ramírez Nasto, Lucía Upna Orcid
    Pisabarro de Lucas, Gerardo Upna Orcid
    Version
    Acceso abierto / Sarbide irekia
    Type
    Contribución a congreso / Biltzarrerako ekarpena
    Version
    Versión publicada / Argitaratu den bertsioa
    Impact
     
     nodoi-noplumx
     
     
     
     
     
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    Abstract
    Pleurotus ostreatus, commonly known as oyster mushroom, is a commercially important edible fungus with interesting biotechnological properties. Quantitative trait loci (QTL) analyses are rare in fungi and little is known about their number, position, and genetic structure. Previous studies of our group have allowed the construction of a genetic linkage map of P. ostreatus var. florida, which ... [++]
    Pleurotus ostreatus, commonly known as oyster mushroom, is a commercially important edible fungus with interesting biotechnological properties. Quantitative trait loci (QTL) analyses are rare in fungi and little is known about their number, position, and genetic structure. Previous studies of our group have allowed the construction of a genetic linkage map of P. ostreatus var. florida, which has provided the basis for performing an efficient QTL analysis. In fact, there is a region of the chromosome VII of P. ostreatus where the most QTLs related to the production and precocity characters have been mapped. These quantitative traits are presumably under the control of a polygenic genetic system and could be associated with some chromosomal regions. The hypothesis of this work is that there is a region in the chromosome VII of protoclon PC15 (monokaryotic parental of the N001 dikaryotic strain) where exist genes which are responsible for the QTLs mentioned above. In order to test this hypothesis, we are developing a molecular QTL analysis through the sequencing of a region with an approximated size of 320 Kbp in chromosome VII (protoclon PC15). For this purpose, a BAC genomic library was constructed and two BAC clones spanning the region of interest are being sequenced. To carry out an efficient computational prediction of protein-coding genes and its annotation on the partial sequences obtained up to date, we have used different Internet resources such as BLASTx, BLASTp, BLASTn, and FGENESH trained on some basidiomycetes genomic data like Phanerochaete chrysosporium and Cryptococcus neoformans (SoftBerry). To our knowledge, this is the firs molecular QTL analysis performed on this edible mushroom. [--]
    Subject
    Pleurotus ostreatus, Quantitative trait loci (QTL) analyses
     
    Publisher
    Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
    Published in
    Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
    Description
    Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
    Departament
    Universidad Pública de Navarra. Departamento de Producción Agraria / Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
     
    URI
    https://hdl.handle.net/2454/27273
    Appears in Collections
    • Comunicaciones y ponencias de congresos DPA - NES Biltzarretako komunikazioak eta txostenak [17]
    • VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI) [60]
    • Comunicaciones y ponencias de congresos - Biltzarrak eta Argitalpenak [580]
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