Identification and functional characterisation of ctr1, a Pleurotus ostreatus gene coding for a copper transporter
Fecha
2006Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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nodoi-noplumx
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Resumen
Copper homeostasis is primordial for life maintenance and especially relevant
for ligning-degrading fungi whose phenol-oxidase enzymes depend on
this micronutrient for their activity. In this paper we report the identification
of a gene (ctr1), coding for a copper transporter in the white rot fungus Pleurotus
ostreatus, in a cDNA library constructed from four-days old vegetative
mycelium gro ...
[++]
Copper homeostasis is primordial for life maintenance and especially relevant
for ligning-degrading fungi whose phenol-oxidase enzymes depend on
this micronutrient for their activity. In this paper we report the identification
of a gene (ctr1), coding for a copper transporter in the white rot fungus Pleurotus
ostreatus, in a cDNA library constructed from four-days old vegetative
mycelium growing in submerged culture. The results presented here indicate
that: (1) ctr1 functionally complements the respiratory deficiency of a yeast
mutant defective in copper transport supporting the transport activity of the
Ctr1 protein; (2) ctr1 transcription is detected in all P. ostreatus developmental
stages (with exception of lamellae) and is negatively regulated by the presence
of copper in the culture media; (3) ctr1 is a single copy gene that maps
to P. ostreatus linkage group III; and (4) the regulatory sequence elements
found in the promoter of ctr1 agree with those found in other copper related
genes described in other systems. These results provide the first description
of a copper transporter in this white rot fungus and open the possibility of
further studies on copper metabolism in higher basidiomyetes. [--]
Materias
Pleurotus ostreatus,
ctr1,
Copper transporter
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila