Enzymatic characterization of a monokaryon population of the edible mushroom, Pleurotus ostreatus with a view to genetic improvement
Fecha
2006Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Contribución a congreso / Biltzarrerako ekarpena
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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nodoi-noplumx
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Resumen
In this work the lignocellulolytic enzymes produced by the edible mushroom
Pleurotus ostreatus var. florida were studied. The objective was to know their
relationship with the degradation of the biopolymers present in the cell wall
of wheat-straw for the purpose of explaining their influence on the production
and quality characters of the fruiting bodies. The following enzymatic
activities w ...
[++]
In this work the lignocellulolytic enzymes produced by the edible mushroom
Pleurotus ostreatus var. florida were studied. The objective was to know their
relationship with the degradation of the biopolymers present in the cell wall
of wheat-straw for the purpose of explaining their influence on the production
and quality characters of the fruiting bodies. The following enzymatic
activities were studied both in solid and submerged culture: Ligninases
(Lignin Peroxidase, Manganese Peroxidase (MnP) and Laccase), Cellulases
(Glucohydrolases, Glucosidases) and Hemicellulases from the group Arabinofuran-
Xylanases (Xylanase, Xilosidase, Glucoronidase, Arabinofuran-Oxidase
and Acetylesterase), cooperating enzymes (Glyoxal Oxidase) and feedback
enzymes (Glucose Oxidase (GOD), Aryl Alcohol Oxidase (AAO),
Tyrosinase (TYR), Veratryl Alcohol Oxydase (VAO), Cellobiose Dehydrogenase
(CDH)). The first studies regarding all the mentioned enzymes were
performed using the dikayon (N001) and the parental monokarion strains
“fast” (PC9) and “slow” (PC15).
The studies on all this whole group of enzymes, which are enough representative
of the lignocellulolytic complex, let to conclude that (both in solid
or submerged culture) the enzymes of major influence in colonizing the natural
substrate and also those whose activity-determination better guarantees
their further mapping were Laccases, MnP, AAO and TYR. Subsequently
these four activities were measured in the monokaryon population being
Laccases and MnP, those yielding the best levels in medium-7 (rich in nitrogen).
In addition both enzymes allow the discrimination between “fast-” or
“slow-” monokaryon strains both in solid medium with several dyes, or in liquid
culture in agitation. The analysis of the enzymatic activities detected in
the assayed conditions, in the population of “fast” or “slow” strains let to the observation that they map in different places where the loci corresponding to
Laccase (pox) and mnp genes are located. These results open the possibility
to design more precise studies that could help to establish a correlation between
the contribution of the genes already described and the activity of the
different ligninolytic enzymes. In addition the results will contribute to know
whether in P. ostreatus genome there are new genes or if they correspond with
locations that regulate these enzymatic activities, or it is a gene that has a role
in the transport system or a kind of effector in the exportation machinery of
the protein to the culture medium. [--]
Materias
Pleurotus ostreatus var. Florida,
Lignocellulolytic enzymes
Editor
Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa
Publicado en
Antonio G. Pisabarro and Lucía Ramírez (eds.): VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI). Pamplona: Universidad Pública de Navarra / Nafarroako Unibertsitate Publikoa, 2006.
Notas
Resumen del poster presentado al VI Meeting on Genetics and Cellular Biology of Basidiomycetes (GCBB-VI), organizado por y celebrado en la Universidad Pública de Navarra el 3-6 de junio de 2005.
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila