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dc.creatorLarraya Reta, Luis Maríaes_ES
dc.creatorFernández San Millán, Aliciaes_ES
dc.creatorAncín Rípodas, Maríaes_ES
dc.creatorFarrán Blanch, Inmaculadaes_ES
dc.creatorVeramendi Charola, Jones_ES
dc.date.accessioned2018-04-10T13:08:14Z
dc.date.available2018-04-10T13:08:14Z
dc.date.issued2015
dc.identifier.issn1860-6768 (Print)
dc.identifier.issn1860-7314 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/28229
dc.description.abstractPlastid genetic engineering represents an attractive system for the production of foreign proteins in plants. Although high expression levels can be achieved in leaf chloroplasts, the results for non-photosynthetic plastids are generally discouraging, mainly due to low transcriptional and translational rates in comparison with chloroplasts. Here, we report the expression of two thioredoxin genes (trx f and m) from the potato plastid genome to study transgene expression in amyloplasts. As expected, the highest transgene expression was detected in the leaf (up to 4.2% of TSP). The Trx protein content in the tuber was approximately 2-3 orders of magnitude lower than in the leaf. However, we demonstrate that a simple post-harvest light treatment of microtubers developed in vitro or soil-grown tubers induces up to 55 times higher accumulation of the recombinant protein in just 7-10 days. The promoter and 5’-UTR of the psbA gene displayed higher light induction than the rrn promoter. After the applied treatment, the Trx f levels in microtubers and soil-grown tubers increased to 0.14% and 0.11% of TSP, respectively. Moreover, tubers stored for 8 months maintained the capacity of increasing the foreign protein levels after the light treatment. Post-harvest cold induction (up to 5 times) at 4 ºC was also detected in microtubers. We conclude that plastid transformation and post-harvest light treatment could be an interesting approach for the production of foreign proteins in potato.en
dc.description.sponsorshipThis work was supported by Grant AGL2010-15107 from the Ministerio de Ciencia e Innovación (Spain). M.A. is a recipient of a FPU fellowship from the Ministerio de Educación, Cultura y Deporte (Spain).en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherWileyen
dc.relation.ispartofBiotechnology Journal, Vol. 10, Issue 11, November 2015, pgs. 1803-1813en
dc.rights© 2015 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheimen
dc.subjectAmyloplasten
dc.subjectMicrotuberen
dc.subjectPlastid transformationen
dc.subjectPotatoen
dc.subjectThioredoxinen
dc.titlePost-harvest light treatment increases expression levels of recombinant proteins in transformed plastids of potato tubersen
dc.typeArtículo / Artikuluaes
dc.typeinfo:eu-repo/semantics/articleen
dc.contributor.departmentIdAB - Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.identifier.doi10.1002/biot.201500028
dc.relation.publisherversionhttps://doi.org/10.1002/biot.201500028
dc.type.versionVersión aceptada / Onetsi den bertsioaes
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen


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