Somatic transposition and meiotically driven elimination of an active helitron family in Pleurotus ostreatus
Fecha
2017Autor
Versión
Acceso abierto / Sarbide irekia
Tipo
Artículo / Artikulua
Versión
Versión publicada / Argitaratu den bertsioa
Impacto
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10.1093/dnares/dsw060
Resumen
Helitrons constitute a superfamily of DNA transposons that were discovered in silico and are
widespread in most eukaryotic genomes. They are postulated to mobilize through a “rollingcircle”
mechanism, but the experimental evidence of their transposition has been described
only recently. Here, we present the inheritance patterns of HELPO1 and HELPO2 helitron families
in meiotically derived pro ...
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Helitrons constitute a superfamily of DNA transposons that were discovered in silico and are
widespread in most eukaryotic genomes. They are postulated to mobilize through a “rollingcircle”
mechanism, but the experimental evidence of their transposition has been described
only recently. Here, we present the inheritance patterns of HELPO1 and HELPO2 helitron families
in meiotically derived progeny of the basidiomycete Pleurotus ostreatus. We found distorted
segregation patterns of HELPO2 helitrons that led to a strong under-representation of
these elements in the progeny. Further investigation of HELPO2 flanking sites showed that
gene conversion may contribute to the elimination of such repetitive elements in meiosis, favouring
the presence of HELPO2 vacant loci. In addition, the analysis of HELPO2 content in a reconstructed
pedigree of subclones maintained under different culture conditions revealed an
event of helitron somatic transposition. Additional analyses of genome and transcriptome data
indicated that P. ostreatus carries active RNAi machinery that could be involved in the control
of transposable element proliferation. Our results provide the first evidence of helitron mobilization
in the fungal kingdom and highlight the interaction between genome defence mechanisms
and invasive DNA. [--]
Materias
Helitrons,
Segregation distortion,
Gene conversion,
Transposable elements,
Basidiomycete
Editor
Oxford University Press
Publicado en
DNA Research, 2017, 24(2), 103–115
Departamento
Universidad Pública de Navarra. Departamento de Producción Agraria /
Nafarroako Unibertsitate Publikoa. Nekazaritza Ekoizpena Saila
Versión del editor
Entidades Financiadoras
This work was supported by Spanish National Research Plan (Projects
AGL2011-30495 and AGL2014-55971-R) and FEDER funds; Public
University of Navarre (http://www.unavarra.es); AB holds a PhD scholarship
from the Public University of Navarre and RC holds a FPI-PhD scholarship
from the Ministry of Economy and Competitiveness.
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