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dc.creatorEsteban Terradillos, Raqueles_ES
dc.creatorRoyo Castillejo, Beatrizes_ES
dc.creatorUrarte Rodríguez, Estíbalizes_ES
dc.creatorZamarreño, Ángel M.es_ES
dc.creatorGarcía Mina, José M.es_ES
dc.creatorMorán Juez, José Fernandoes_ES
dc.date.accessioned2018-09-04T06:43:50Z
dc.date.available2018-09-04T06:43:50Z
dc.date.issued2016
dc.identifier.issn1664-462X (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/30440
dc.description.abstractWe aimed to identify the early stress response and plant performance of Medicago truncatula growing in axenic medium with ammonium or urea as the sole source of nitrogen, with respect to nitrate-based nutrition. Biomass measurements, auxin content analyses, root system architecture (RSA) response analyses, and physiological parameters were determined. Both ammonium and ureic nutrition severely affected the RSA, resulting in changes in the main elongation rate, lateral root development, and insert position from the root base. The auxin content decreased in both urea- and ammonium-treated roots; however, only the ammonium-treated plants were affected at the shoot level. The analysis of chlorophyll a fluorescence transients showed that ammonium affected photosystem II, but urea did not impair photosynthetic activity. Superoxide dismutase isoenzymes in the plastids were moderately affected by urea and ammonium in the roots. Overall, our results showed that low N doses from different sources had no remarkable effects on M. truncatula, with the exception of the differential phenotypic root response. High doses of both ammonium and urea caused great changes in plant length, auxin contents and physiological measurements. Interesting correlations were found between the shoot auxin pool and both plant length and the “performance index” parameter, which is obtained from measurements of the kinetics of chlorophyll a fluorescence. Taken together, these data demonstrate that both the indole-3-acetic acid pool and performance index are important components of the response of M. truncatula under ammonium or urea as the sole N source.en
dc.description.sponsorshipThis work was supported by the grants AGL2010-16167 and AGL2014-52396-from the Spanish Ministry of Economy and Competitiviness-Mineco. BR is a holder of a PhD fellowship from the Mineco. RE received a JAE-Doc-2011-046 contract from the Spanish Research Council (CSIC) of the programme "Junta para la Ampliación de Estudios" co-financed by the European Social Fund.en
dc.format.extent14 p.
dc.format.mimetypeapplication/pdfen
dc.format.mimetypeapplication/zipen
dc.language.isoengen
dc.publisherFrontiers Mediaen
dc.relation.ispartofFrontiers in Plant Science, 7:140en
dc.rights© 2016 Esteban, Royo, Urarte, Zamarreño, Garcia-Mina and Moran. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAmmoniumen
dc.subjectAuxinen
dc.subjectOJIP curveen
dc.subjectNitrateen
dc.subjectRoot system architectureen
dc.subjectUreaen
dc.titleBoth free indole-3-acetic acid and photosynthetic performance are important players in the response of Medicago truncatula to urea and ammonium nutrition under axenic conditionsen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB - Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.3389/fpls.2016.00140
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/AGL2014-52396en
dc.relation.publisherversionhttps://doi.org/10.3389/fpls.2016.00140
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


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© 2016 Esteban, Royo, Urarte, Zamarreño, Garcia-Mina and Moran. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as © 2016 Esteban, Royo, Urarte, Zamarreño, Garcia-Mina and Moran. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.