Show simple item record

dc.creatorQuiles Puchalt, Nuriaes_ES
dc.creatorTormo Más, María Ángeleses_ES
dc.creatorCampoy Sánchez, Susanaes_ES
dc.creatorToledo Arana, Alejandroes_ES
dc.creatorMonedero, Vicentees_ES
dc.creatorLasa Uzcudun, Íñigoes_ES
dc.creatorNovick, Richard P.es_ES
dc.creatorChristie, Gail E.es_ES
dc.creatorPenadés, José R.es_ES
dc.date.accessioned2018-09-06T12:20:13Z
dc.date.available2018-09-06T12:20:13Z
dc.date.issued2013
dc.identifier.issn0305-1048 (Print)
dc.identifier.issn1362-4962 (Electronic)
dc.identifier.urihttps://hdl.handle.net/2454/30528
dc.description.abstractThe propagation of bacteriophages and other mobile genetic elements requires exploitation of the phage mechanisms involved in virion assembly and DNA packaging. Here, we identified and characterized four different families of phage-encoded proteins that function as activators required for transcription of the late operons (morphogenetic and lysis genes) in a large group of phages infecting Gram-positive bacteria. These regulators constitute a super-family of proteins, here named late transcriptional regulators (Ltr), which share common structural, biochemical and functional characteristics and are unique to this group of phages. They are all small basic proteins, encoded by genes present at the end of the early gene cluster in their respective phage genomes and expressed under cI repressor control. To control expression of the late operon, the Ltr proteins bind to a DNA repeat region situated upstream of the ter S gene, activating its transcription. This involves the C-terminal part of the Ltr proteins, which control specificity for the DNA repeat region. Finally, we show that the Ltr proteins are the only phage-encoded proteins required for the activation of the packaging and lysis modules. In summary, we provide evidence that phage packaging and lysis is a conserved mechanism in Siphoviridae infecting a wide variety of Gram-positive bacteria.en
dc.description.sponsorshipFunding for open access charge: Ministerio de Ciencia e Innovación (MICINN) [Consolider-Ingenio CSD2009-00006, BIO2011-30503-C02-01 and Eranet-pathogenomics PIM2010EPA-00606 to J.R.P]; Cardenal Herrera-CEU University [Copernicus-Santander program to J.R.P.]; Insituto Nacional de Investigaciones Agrarias (INIA) [DR08-0093 to M.A.T-M.]; National Institute of Health [R56AI081837 to G.E.C, R01AI022159-23A2 to R.P.N.].en
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherOxford University Pressen
dc.relation.ispartofNucleic Acids Research, 2013, Vol. 41, No. 15en
dc.rights© The Author(s) 2013. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercialre-use, please contact journals.permissions@oup.comen
dc.rights.urihttps://creativecommons.org/licenses/by-nc/3.0/
dc.subjectLate operonsen
dc.subjectGram-positive bacteriaen
dc.subjectLate transcriptional regulatorsen
dc.subjectPhage packaging and lysisen
dc.titleA super-family of transcriptional activators regulates bacteriophage packaging and lysis in Gram-positive bacteriaen
dc.typeinfo:eu-repo/semantics/articleen
dc.typeArtículo / Artikuluaes
dc.contributor.departmentIdAB. Instituto de Agrobiotecnología / Agrobioteknologiako Institutuaes
dc.rights.accessRightsinfo:eu-repo/semantics/openAccessen
dc.rights.accessRightsAcceso abierto / Sarbide irekiaes
dc.identifier.doi10.1093/nar/gkt508
dc.relation.projectIDinfo:eu-repo/grantAgreement/MICINN//BIO2011-30503-C02-02/ES/en
dc.relation.publisherversionhttps://doi.org/10.1093/nar/gkt508
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.type.versionVersión publicada / Argitaratu den bertsioaes


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record

© The Author(s) 2013. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercialre-use, please contact journals.permissions@oup.com
Except where otherwise noted, this item's license is described as © The Author(s) 2013. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercialre-use, please contact journals.permissions@oup.com

El Repositorio ha recibido la ayuda de la Fundación Española para la Ciencia y la Tecnología para la realización de actividades en el ámbito del fomento de la investigación científica de excelencia, en la Línea 2. Repositorios institucionales (convocatoria 2020-2021).
Logo MinisterioLogo Fecyt